Medizinische Universität Graz - Research portal
Selected Publication:
SHR Neuro Cancer Cardio Lipid Metab Microb
Janowski, R; Scanu, S; Niessing, D; Madl, T.
Crystal and solution structural studies of mouse phospholipid hydroperoxide glutathione peroxidase 4.
Acta Crystallogr F Struct Biol Commun. 2016; 72(Pt 10):743-749
Doi: 10.1107/S2053230X16013686
[OPEN ACCESS]
Web of Science
PubMed
FullText
FullText_MUG
- Leading authors Med Uni Graz
- Madl Tobias
- Altmetrics:
- Dimensions Citations:
- Plum Analytics:
- Scite (citation analytics):
- Abstract:
- The mammalian glutathione peroxidase (GPx) family is a key component of the cellular antioxidative defence system. Within this family, GPx4 has unique features as it accepts a large class of hydroperoxy lipid substrates and has a plethora of biological functions, including sperm maturation, regulation of apoptosis and cerebral embryogenesis. In this paper, the structure of the cytoplasmic isoform of mouse phospholipid hydroperoxide glutathione peroxidase (O70325-2 GPx4) with selenocysteine 46 mutated to cysteine is reported solved at 1.8 Å resolution using X-ray crystallography. Furthermore, solution data of an isotope-labelled GPx protein are presented.
- Find related publications in this database (using NLM MeSH Indexing)
- Amino Acid Sequence -
- Animals -
- Cloning, Molecular -
- Crystallography, X-Ray -
- Cysteine - chemistry
- Cysteine - metabolism
- Escherichia coli - genetics
- Escherichia coli - metabolism
- Gene Expression -
- Glutathione Peroxidase - chemistry
- Glutathione Peroxidase - genetics
- Glutathione Peroxidase - metabolism
- Mice -
- Models, Molecular -
- Mutation -
- Plasmids - chemistry
- Plasmids - metabolism
- Protein Conformation, alpha-Helical -
- Protein Conformation, beta-Strand -
- Protein Interaction Domains and Motifs -
- Recombinant Proteins - chemistry
- Recombinant Proteins - genetics
- Recombinant Proteins - metabolism
- Selenocysteine - chemistry
- Selenocysteine - metabolism
- Substrate Specificity -
- Find related publications in this database (Keywords)
- phospholipid hydroperoxide glutathione peroxidase 4
- reactive oxidative species
- NMR spectroscopy
- small-angle X-ray scattering