Medizinische Universität Graz Austria/Österreich - Forschungsportal - Medical University of Graz
Gewählte Publikation:
Malle, E; Ibovnik, A; Leis, HJ; Kostner, GM; Verhallen, PF; Sattler, W.
Lysine modification of LDL or lipoprotein(a) by 4-hydroxynonenal or malondialdehyde decreases platelet serotonin secretion without affecting platelet aggregability and eicosanoid formation.
Arterioscler Thromb Vasc Biol. 1995; 15(3):377-384
Doi: 10.1161/01.ATV.15.3.377
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- Führende Autor*innen der Med Uni Graz
- Malle Ernst
- Co-Autor*innen der Med Uni Graz
- Kostner Gerhard
- Leis Hans-Joerg
- Sattler Wolfgang
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- Abstract:
- The effects of lysine-modified atherogenic plasma lipoproteins, known to be constituents of human atherosclerotic plaques, were studied on platelet function in vitro. LDL and lipoprotein(a) [Lp(a)] modified with secondary breakdown products of lipid peroxidation (4-hydroxy-2,3-trans-nonenal [HNE] 0.1 to 10 mmol/L or malondialdehyde [MDA] 1 to 50 mmol/L) induced neither spontaneous platelet aggregation nor secretion of 5-hydroxytryptamine (5-HT) from platelet aminestorage granules. Incubation of platelets with HNE- or MDA-modified LDL or Lp(a) (up to 1200 micrograms protein/mL) prior to thrombin (0.2 U/mL)- or collagen (2 micrograms/mL)-induced aggregation did not enhance platelet aggregability or formation of eicosanoids, ie, thromboxane A2 or prostaglandins E2 and F2 alpha. In contrast to native lipoproteins, HNE- or MDA-modified LDL and Lp(a) (approximately 20% to 30% of total apolipoprotein lysine residues modified) exerted a pronounced dose-dependent inhibition of 5-HT release from activated platelets in the following order: HNE LDL (50%) > HNE Lp(a) (40%) > MDA LDL (20%) > MDA Lp(a) (5%). Preincubation of human blood platelets with acetylated LDL or Lp(a) (approximately 60% to 70% of total lysine residues modified) prior to aggregation impaired serotonin secretion by 50% compared with native LDL or Lp(a). These findings suggest that the interaction of platelets with aldehyde-modified atherogenic plasma lipoproteins should not necessarily be considered as proatherogenic with respect to the effects observed in our in vitro studies.
- Find related publications in this database (using NLM MeSH Indexing)
- Acetylation -
- Aldehydes - pharmacology
- Blood Platelets - metabolism
- Eicosanoids - biosynthesis
- Gas Chromatography-Mass Spectrometry - biosynthesis
- Humans - biosynthesis
- Lipoprotein(a) - metabolism
- Lipoproteins, LDL - metabolism
- Lysine - metabolism
- Malondialdehyde - pharmacology
- Platelet Aggregation - drug effects
- Serotonin - metabolism
- Find related publications in this database (Keywords)
- Platelet Lipoprotein Interaction
- Acetylation
- Dense Granule Secretion
- Eicosanoids
- Gas Chromatography Mass Spectrometry