Medizinische Universität Graz Austria/Österreich - Forschungsportal - Medical University of Graz
Gewählte Publikation:
SHR Neuro Krebs Kardio Lipid Stoffw Microb
Wallace, PW; Haernvall, K; Ribitsch, D; Zitzenbacher, S; Schittmayer, M; Steinkellner, G; Gruber, K; Guebitz, GM; Birner-Gruenberger, R.
PpEst is a novel PBAT degrading polyesterase identified by proteomic screening of Pseudomonas pseudoalcaligenes.
Appl Microbiol Biotechnol. 2017; 101(6):2291-2303
Doi: 10.1007/s00253-016-7992-8
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- Führende Autor*innen der Med Uni Graz
- Birner-Grünberger Ruth
- Wallace Pal William
- Co-Autor*innen der Med Uni Graz
- Schittmayer-Schantl Matthias
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- Abstract:
- A novel esterase, PpEst, that hydrolyses the co-aromatic-aliphatic polyester poly(1,4-butylene adipate-co-terephthalate) (PBAT) was identified by proteomic screening of the Pseudomonas pseudoalcaligenes secretome. PpEst was induced by the presence of PBAT in the growth media and had predicted arylesterase (EC 3.1.1.2) activity. PpEst showed polyesterase activity on both whole and milled PBAT film releasing terephthalic acid and 4-(4-hydroxybutoxycarbonyl)benzoic acid while end product inhibition by 4-(4-hydroxybutoxycarbonyl)benzoic acid was observed. Modelling of an aromatic polyester mimicking oligomer into the PpEst active site indicated that the binding pocket could be big enough to accommodate large polymers. This is the first report of a PBAT degrading enzyme being identified by proteomic screening and shows that this approach can contribute to the discovery of new polymer hydrolysing enzymes. Moreover, these results indicate that arylesterases could be an interesting enzyme class for identifications of polyesterases.
- Find related publications in this database (using NLM MeSH Indexing)
- Bacterial Proteins - chemistry
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- Biodegradable Plastics - metabolism
- Carboxylic Ester Hydrolases - chemistry
- Carboxylic Ester Hydrolases - genetics
- Carboxylic Ester Hydrolases - metabolism
- Catalytic Domain -
- Gene Expression -
- Models, Molecular -
- Phthalic Acids - chemistry
- Phthalic Acids - metabolism
- Polyesters - chemistry
- Polyesters - metabolism
- Protein Binding -
- Proteomics -
- Pseudomonas pseudoalcaligenes - enzymology
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- Find related publications in this database (Keywords)
- Proteomics
- Poly(1,4-butylene adipate-co-terephthalate) (PBAT)
- Secretome
- Polymer degradation
- Polyesterase
- Arylesterase