Medizinische Universität Graz - Research portal
Selected Publication:
SHR Neuro Cancer Cardio Lipid Metab Microb
Guan, X; Dei-Anane, G; Liang, R; Gross, ML; Nickkholgh, A; Kern, M; Ludwig, J; Zeier, M; Büchler, MW; Schmidt, J; Schemmer, P.
Donor preconditioning with taurine protects kidney grafts from injury after experimental transplantation.
J Surg Res. 2008; 146(1):127-134
Doi: 10.1016/j.jss.2007.06.014
Web of Science
PubMed
FullText
FullText_MUG
- Leading authors Med Uni Graz
- Schemmer Peter
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- Abstract:
- Ischemia/reperfusion injury is a major problem in clinical transplantation (Tx). Taurine has been shown to protect liver grafts from ischemia/reperfusion injury after Tx. Thus, this study was designed to evaluate its effect on kidney grafts after transplantation. Various concentrations of taurine were infused before donor nephrectomy (1.5 mL; 30, 100, 300 mM). Controls were given the same volume of Ringers' solution. Subsequently, grafts were cold-stored for 19 h in histidine-tryptophan-ketoglutarate solution and transplanted. Six hours after Tx, graft function and injury were assessed with blood urea nitrogen/creatinine and aspartate aminotransferase/lactate dehydrogenase. Graft biopsies were taken to evaluate tubular damage, caspase-3, superoxide dismutase, and heat shock protein 72 (HSP-72) to index necrosis, apoptosis, antioxidative capacity, and regeneration, respectively. Taurine significantly decreased blood urea nitrogen, creatinine, aspartate aminotransferase, and lactate dehydrogenase in a dose-dependent manner to up to 71%, 69%, 51%, and 53% of controls, respectively. Further, tubular damage and caspase-3 expression decreased to 44% and 18% of control values (P < 0.01), while superoxide dismutase and heat shock protein 72 expression increased by 95% and 77% of controls, respectively (P < 0.05). This study demonstrates that donor preconditioning with taurine protects kidney grafts from injury (apoptosis, necrosis), improves graft function, and increases the regenerative potential most likely via mechanisms including antioxidation.
- Find related publications in this database (using NLM MeSH Indexing)
- Animals -
- Apoptosis - drug effects
- Biopsy -
- Caspase 3 - metabolism
- Dose-Response Relationship, Drug -
- Female -
- Graft Rejection - pathology
- Graft Rejection - prevention & control
- HSP72 Heat-Shock Proteins - metabolism
- Kidney - drug effects
- Kidney - metabolism
- Kidney - pathology
- Kidney Transplantation - methods
- Kidney Transplantation - pathology
- Models, Animal -
- Necrosis - pathology
- Necrosis - prevention & control
- Rats -
- Rats, Sprague-Dawley -
- Reperfusion Injury - pathology
- Reperfusion Injury - prevention & control
- Superoxide Dismutase - metabolism
- Taurine - pharmacology
- Find related publications in this database (Keywords)
- apoptosis
- kidney transplantation
- regeneration
- reperfusion injury
- taurine
- tubular damage