Medizinische Universität Graz - Research portal
Selected Publication:
SHR Neuro Cancer Cardio Lipid Metab Microb
Liang, R; Bruns, H; Kincius, M; Lin, T; Ludwig, J; Dei-Anane, G; Guan, X; Gebhard, MM; Büchler, MW; Schemmer, P.
Danshen protects liver grafts from ischemia/reperfusion injury in experimental liver transplantation in rats.
Transpl Int. 2009; 22(11):1100-1109
Doi: 10.1111/j.1432-2277.2009.00925.x
Web of Science
PubMed
FullText
FullText_MUG
- Leading authors Med Uni Graz
- Schemmer Peter
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- Abstract:
- Reperfusion injury remains one of the major problems in transplantation. Free radicals and disturbance of microcirculation are the supposed main contributors. Recent evidence shows that Danshen, a traditional Chinese drug used in vascular diseases, can scavenge radicals and improve microcirculation. This study investigates its effect on liver transplantation (LTx). Before organ recovery, female Sprague-Dawley rats (210-240 g) received intravenous Danshen or the same volume of Ringer solution as control. LTx was performed after 1 h of cold storage. Microperfusion, leukocyte-endothelium interaction and latex-bead phagocytosis were evaluated with in vivo microscopy. Survival, transaminases and histology were assessed. Immunohistology was used for TNF-alpha levels. anova and Fisher's exact test were employed for statistical analyses as appropriate. Survival increased from 60% in controls to 100% (P < 0.05). AST and LDH decreased from 3969 +/- 1255 U/l and 15444 +/- 5148 U/l in controls to 1236 +/- 410 U/l and 5039 +/- 1594 U/l, respectively (P < 0.05). In vivo microscopy revealed decreased leukocyte-adherence and increased blood flow velocity in sinusoidal zones after administration of Danshen (P < 0.05), while latex-bead phagocytosis was found in 60% of controls (P < 0.05). The TNF-alpha index decreased from 2.08 +/- 0.09 in controls to 1.09 +/- 0.09 (P < 0.05). This study clearly demonstrates hepatoprotective effects after experimental LTx, which can be explained via anti-oxidative effects, improved microcirculation and decreased Kupffer cell activation.
- Find related publications in this database (using NLM MeSH Indexing)
- Animals -
- Cell Adhesion - drug effects
- Drug Evaluation, Preclinical -
- Drugs, Chinese Herbal - pharmacology
- Drugs, Chinese Herbal - therapeutic use
- Endothelium, Vascular - physiopathology
- Female -
- Free Radical Scavengers - pharmacology
- Free Radical Scavengers - therapeutic use
- Kupffer Cells - drug effects
- Kupffer Cells - immunology
- Leukocytes - physiology
- Liver - blood supply
- Liver - ultrastructure
- Liver Transplantation -
- Microcirculation - drug effects
- Microspheres -
- Oxidative Stress - drug effects
- Phagocytosis - drug effects
- Phytotherapy -
- Postoperative Complications - prevention & control
- Rats -
- Rats, Sprague-Dawley -
- Reperfusion Injury - etiology
- Reperfusion Injury - prevention & control
- Salvia miltiorrhiza -
- Tumor Necrosis Factor-alpha - analysis
- Find related publications in this database (Keywords)
- Danshen
- liver transplantation
- microcirculation
- oxidative stress
- reperfusion injury