Medizinische Universität Graz Austria/Österreich - Forschungsportal - Medical University of Graz
Gewählte Publikation:
SHR Neuro Krebs Kardio Lipid Stoffw Microb
Mercuri, A; Wu, S; Stranzinger, S; Mohr, S; Salar-Behzadi, S; Bresciani, M; Fröhlich, E.
In vitro and in silico characterisation of Tacrolimus released under biorelevant conditions.
Int J Pharm. 2016; 515(1-2):271-280
Doi: 10.1016/j.ijpharm.2016.10.020
Web of Science
PubMed
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- Führende Autor*innen der Med Uni Graz
- Fröhlich Eleonore
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- Abstract:
- This work aims to better understand the in vivo behaviour of modified release (MR) formulations (Envarsus® tablets and Advagraf® capsules) using in vitro properties of tacrolimus and in silico simulations. The in silico concentration profiles of tacrolimus released from the MR formulations were predicted after building a three compartments PK model with GastroPlus™, and using the experimentally determined in vitro physico-chemical properties as input parameters. In vitro-in vivo correlations (IVIVC) were obtained after deconvolution of in vivo data from a clinical trial. The IVIVC showed that the in vitro dissolution was faster than the in vivo deconvoluted dissolution for Advagraf®, while the in vitro dissolution was slightly slower than the in vivo deconvoluted dissolution for Envarsus®. Population PK simulation showed that variability in the simulation was lower for Envarsus® compared to Advagraf®. The in silico predicted preferential absorption sites were the proximal and distal tract for Advagraf® and Envarsus®, respectively. The integration of experimental in vitro solubility, permeability and biorelevant dissolution data allowed to generate in silico tacrolimus concentrations for two different MR formulations. This permitted to compare the two formulations in a single PK profile, in a simulated population PK study and with respect to their absorption sites. Copyright © 2016 Elsevier B.V. All rights reserved.
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- Find related publications in this database (Keywords)
- Biorelevant dissolution
- PK modelling
- Tacrolimus
- Permeability
- Solubility