Medizinische Universität Graz Austria/Österreich - Forschungsportal - Medical University of Graz
Gewählte Publikation:
SHR Neuro Krebs Kardio Lipid Stoffw Microb
Eberl, A; Altendorfer-Kroath, T; Kollmann, D; Birngruber, T; Sinner, F; Raml, R; Magnes, C.
Determination of (2)H-enrichment of rat brain interstitial fluid and rat plasma by headspace-gas-chromatography - quadrupole-mass-spectrometry.
Anal Biochem. 2016; 509(3):130-134
Doi: 10.1016/j.ab.2016.07.010
Web of Science
PubMed
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- Co-Autor*innen der Med Uni Graz
- Birngruber Thomas
- Sinner Frank Michael
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- Abstract:
- (2)H2O as nonradioactive, stable marker substance is commonly used in preclinical and clinical studies and the precise determination of (2)H2O concentration in biological samples is crucial. However, aside from isotope ratio mass spectrometry (IRMS), only a very limited number of methods to accurately measure the (2)H2O concentration in biological samples are routinely established until now. In this study, we present a straightforward method to accurately measure (2)H-enrichment of rat brain interstitial fluid (ISF) and rat plasma to determine the relative recovery of a cerebral open flow microperfusion (cOFM) probe, using headspace-gas-chromatography - quadrupole-mass-spectrometry. This method is based on basic-catalyzed hydrogen/deuterium exchange in acetone and detects the (2)H-labelled acetone directly by the headspace GC-MS. Small sample volumes and limited number of preparation steps make this method highly competitive. It has been fully validated. (2)H enriched to 8800 ppm in plasma showed an accuracy of 98.9% and %Relative Standard Deviation (RSD) of 3.1 with n = 18 over three days and with two operators. Similar performance was obtained for cerebral ISF enriched to 1100 ppm (accuracy: 96.5%, %RSD: 3.1). With this highly reproducible method we demonstrated the successful employment of (2)H2O as performance marker for a cOFM probe. Copyright © 2016. Published by Elsevier Inc.
- Find related publications in this database (using NLM MeSH Indexing)
- Animals -
- Brain - metabolism
- Deuterium Exchange Measurement - methods
- Deuterium Oxide - analysis
- Deuterium Oxide - pharmacokinetics
- Deuterium Oxide - pharmacology
- Extracellular Fluid - chemistry
- Extracellular Fluid - metabolism
- Gas Chromatography-Mass Spectrometry - methods
- Rats -
- Find related publications in this database (Keywords)
- (H2O)-H-2
- Headspace GC-MS
- Validation
- Interstitial fluid
- Plasma
- Cerebral open flow microperfusion