Medizinische Universität Graz Austria/Österreich - Forschungsportal - Medical University of Graz
Gewählte Publikation:
SHR Neuro Krebs Kardio Lipid Stoffw Microb
Oberauner-Wappis, L; Loibner, M; Viertler, C; Groelz, D; Wyrich, R; Zatloukal, K.
Protocol for HER2 FISH determination on PAXgene-fixed and paraffin-embedded tissue in breast cancer.
Int J Exp Pathol. 2016; 97(2):202-206
Doi: 10.1111/iep.12185
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- Führende Autor*innen der Med Uni Graz
- Oberauner-Wappis Lisa
- Zatloukal Kurt
- Co-Autor*innen der Med Uni Graz
- Loibner Martina
- Viertler Christian
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- Abstract:
- Molecular diagnostics in personalized medicine increasingly relies on the combination of a variety of analytical technologies to characterize individual diseases and to select patients for targeted therapies. The gold standard for tissue-based diagnostics is fixation in formalin and embedding in paraffin, which results in excellent preservation of morphology but negatively impacts on a variety of molecular assays. The formalin-free, non-cross-linking PAXgene tissue system preserves morphology in a similar way to formalin, but also preserves biomolecules essentially in a similar way to cryopreservation, which markedly widens the spectrum, sensitivity and accuracy of molecular analytics. In this study, we have developed and tested a protocol for PAXgene-fixed and paraffin-embedded tissues for fluorescent in situ hybridization (FISH). The implementation of a 24-h formalin postfixation step of slides from PAXgene-fixed and paraffin-embedded tissues allowed us to use the assays approved for formalin-fixed and paraffin-embedded tissues. The equivalence of the methodologies was demonstrated by FISH analysis of HER2 amplification in breast cancer cases. The 24-h postfixation step of the slides used for FISH can be well integrated in the routine diagnostic workflow and allows the remaining PAXgene-fixed and paraffin-embedded tissue to be used for further molecular testing. © 2016 The Authors. International Journal of Experimental Pathology published by John Wiley & Sons Ltd on behalf of Company of the International Journal of Experimental Pathology (CIJEP).
- Find related publications in this database (using NLM MeSH Indexing)
- Adult -
- Aged -
- Aged, 80 and over -
- Breast Neoplasms - chemistry
- Breast Neoplasms - genetics
- Clinical Protocols -
- Female -
- Fixatives -
- Formaldehyde -
- Gene Amplification -
- Humans -
- In Situ Hybridization, Fluorescence - methods
- Middle Aged -
- Paraffin Embedding - methods
- Receptor, ErbB-2 - analysis
- Receptor, ErbB-2 - genetics
- Tissue Fixation - methods
- Find related publications in this database (Keywords)
- breast cancer
- FISH
- HER2
- molecular diagnostics
- Postfixation of PAXgene tissue