Medizinische Universität Graz Austria/Österreich - Forschungsportal - Medical University of Graz
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SHR Neuro Krebs Kardio Lipid Stoffw Microb
Kriegshäuser, G; Fabjani, G; Ziegler, B; Zöchbauer-Müller, S; End, A; Zeillinger, R.
Biochip-based detection of KRAS mutation in non-small cell lung cancer.
Int J Mol Sci. 2011; 12(12): 8530-8538.
Doi: 10.3390/ijms12128530
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- Führende Autor*innen der Med Uni Graz
- Kriegshäuser Gernot
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- Abstract:
- This study is aimed at evaluating the potential of a biochip assay to sensitively detect KRAS mutation in DNA from non-small cell lung cancer (NSCLC) tissue samples. The assay covers 10 mutations in codons 12 and 13 of the KRAS gene, and is based on mutant-enriched PCR followed by reverse-hybridization of biotinylated amplification products to an array of sequence-specific probes immobilized on the tip of a rectangular plastic stick (biochip). Biochip hybridization identified 17 (21%) samples to carry a KRAS mutation of which 16 (33%) were adenocarcinomas and 1 (3%) was a squamous cell carcinoma. All mutations were confirmed by DNA sequencing. Using 10 ng of starting DNA, the biochip assay demonstrated a detection limit of 1% mutant sequence in a background of wild-type DNA. Our results suggest that the biochip assay is a sensitive alternative to protocols currently in use for KRAS mutation testing on limited quantity samples.
- Find related publications in this database (using NLM MeSH Indexing)
- Adult -
- Aged -
- Aged, 80 and over -
- Biomarkers, Tumor - genetics
- Carcinoma, Non-Small-Cell Lung - diagnosis Carcinoma, Non-Small-Cell Lung - genetics
- Case-Control Studies -
- Female -
- Humans -
- Lung Neoplasms - diagnosis Lung Neoplasms - genetics
- Male -
- Middle Aged -
- Mutation -
- Oligonucleotide Array Sequence Analysis -
- Proto-Oncogene Proteins - genetics
- ras Proteins - genetics
- Find related publications in this database (Keywords)
- non-small cell lung cancer
- KRAS
- mutation detection
- biochip hybridization