Medizinische Universität Graz Austria/Österreich - Forschungsportal - Medical University of Graz
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SHR Neuro Krebs Kardio Lipid Stoffw Microb
Kriegshäuser, G; Cullinane, A; Kuechler, E; Skern, T.
Denatured virion protein 1 of equine rhinitis B virus 1 contains authentic B-cell epitopes recognised in an enzyme-linked immunosorbent assay--short communication.
Acta Vet Hung. 2008; 56(2): 265-270.
Doi: 10.1556/AVet.56.2008.2.14
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- Kriegshäuser Gernot
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- Abstract:
- Equine rhinitis B virus 1 (ERBV1), genus Erbovirus, family Picornaviridae, is a pathogen of horses which causes clinical and subclinical infection of the upper respiratory tract in horses. The virus is widespread in European horse populations and the current standard method for the detection of antibody against ERBV1 is by virus neutralisation (VN). VN tests, however, are labour-intensive and time-consuming, require tissue culture facilities, and generally do not provide same-day results. In this study, a protocol for the high-level expression and purification of recombinant virion protein 1 (rVP1) was established using metal-chelate affinity chromatography under denaturing condition. When used as a coating antigen in a prototype enzyme-linked immunosorbent assay (ELISA), denatured rVP1 was recognised by ERBV1 antibody present in horse serum. This finding suggests that denatured rVP1 is a promising candidate for the development of an ELISA to be used in the routine laboratory diagnosis of ERBV1 infection in horses.
- Find related publications in this database (using NLM MeSH Indexing)
- Animals -
- Antibodies, Viral - blood
- Antigens, Viral - chemistry
- Enzyme-Linked Immunosorbent Assay - veterinary
- Epitopes, B-Lymphocyte - analysis
- Erbovirus - immunology Erbovirus - isolation & purification
- Horse Diseases - virology
- Horses -
- Picornaviridae Infections - veterinary Picornaviridae Infections - virology
- Respiratory Tract Infections - veterinary Respiratory Tract Infections - virology
- Find related publications in this database (Keywords)
- capsid protein
- ELISA
- equine rhinitis B virus 1
- serodiagnosis