Medizinische Universität Graz Austria/Österreich - Forschungsportal - Medical University of Graz
Gewählte Publikation:
SHR Neuro Krebs Kardio Lipid Stoffw Microb
Löhr, M; Molcanyi, M; Stenzel, W; Seifert, H; Tzouras, G; Röhn, G; Mohseni, D; Hampl, JA.
A novel experimental in vivo model of cerebral immunomodulation induced by inactivated Staphylococcus epidermidis.
J Neurosci Methods. 2012; 203(1): 89-95.
Doi: 10.1016/j.jneumeth.2011.09.016
Web of Science
PubMed
FullText
FullText_MUG
- Co-Autor*innen der Med Uni Graz
- Molcanyi Marek
- Altmetrics:
- Dimensions Citations:
- Plum Analytics:
- Scite (citation analytics):
- Abstract:
- The genesis and appropriate treatment of neuroinflammation in various infectious and non-infectious disorders of the central nervous system is still a matter of debate. We introduce an alternative and simple experimental model for the investigation of the cellular inflammatory response to bacterial antigens by stereotactic intracerebral injection of heat-inactivated Staphylococcus epidermidis (HISE). HISE-injection resulted in well-circumscribed intraparenchymal deposits encompassed by an early micro- and astroglial response and a selective but sustained opening of the blood-brain barrier (BBB). After 24h, the HISE collections were densely infiltrated by granulocytes and few circumjacent macrophages that became the predominating immunocompetent cell type from day 4 on. CD8a+ lymphocytes peaked at day 4, whereas CD4+ and CD20+ lymphocytes increased gradually in number, developing a scattered infiltrate until day 17, indicating the initiation of an adaptive immune response. MHC class II presenting cells were abundantly recruited from day 1 and eventually shaped an increasingly dense accumulation within the lesion. Intracerebral HISE administration provides a controlled, highly reproducible and well defined influx of immunocompetent cells across the BBB leading to a distinct and condensed inflammatory reaction. The technique is straightforward, easily feasible and may significantly enable further investigations of the initiation, maintenance and therapeutic modulation of acute neuroinflammation. Copyright © 2011 Elsevier B.V. All rights reserved.
- Find related publications in this database (using NLM MeSH Indexing)
- Animals -
- Disease Models, Animal -
- Encephalitis - immunology Encephalitis - microbiology Encephalitis - pathology
- Immunomodulation - immunology
- Male -
- Rats -
- Rats, Wistar -
- Staphylococcus epidermidis - immunology
- Find related publications in this database (Keywords)
- Neuroinflammation
- Experimental model
- Central nervous system
- Staphylococcus epidermidis
- Immunomodulation
- Blood brain barrier