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SHR Neuro Cancer Cardio Lipid Metab Microb
Zani, A; Zani-Ruttenstock, E; Peyvandi, F; Lee, C; Li, B; Pierro, A.
A spectrum of intestinal injury models in neonatal mice.
Pediatr Surg Int. 2016; 32(1):65-70
Doi: 10.1007/s00383-015-3813-x
Web of Science
PubMed
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- Co-authors Med Uni Graz
- Ruttenstock Elke Maria
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- Abstract:
- To compare the degree of necrotizing enterocolitis (NEC)-like damage under different stress conditions in neonatal mice. 5-day-old C57BL/6 mice were assigned to: (A) breastfed and no stress factors; (B) breastfed+maternal separation (3 h daily); (C) breastfed+hypoxia+lipopolysaccharide (LPS-4 mg/kg/day); (D) hyperosmolar formula+hypoxia+LPS. Mice were killed at 9 days of life. Ileum and colon were stained for hematoxylin/eosin and blindly assessed. A scoring ≥2 was considered NEC. Data were compared using one-way ANOVA and reported as median (range). Ileum-Mucosal injury was mild in group B (0.0-1). Hypoxia+LPS induced greater injury in group C (1.6, 1-2.5; p < 0.0001 to B) and D (2, 0.5-3.5; p < 0.0001 to B). There were no differences between group C and D (p = n.s.). There were no cases of NEC in group A or B, whereas NEC was present in 36 % group C and 68 % group D mice. Colon-a similar degree of mucosal injury was observed among group B (2, 1-3), C (1.7, 0-3) and D (1.5, 1-3; p = n.s.). NEC was present in 75 % of group B, 50 % of group C and 86 % of group D. These models establish a spectrum of intestinal injury and are useful to investigate the variability of neonatal intestinal diseases, such as NEC.
- Find related publications in this database (using NLM MeSH Indexing)
- Analysis of Variance -
- Animals -
- Animals, Newborn -
- Disease Models, Animal -
- Enterocolitis, Necrotizing - complications
- Enterocolitis, Necrotizing - physiopathology
- Hypoxia - physiopathology
- Lipopolysaccharides - administration & dosage
- Maternal Deprivation -
- Mice -
- Mice, Inbred C57BL -
- Stress, Physiological - physiology
- Stress, Psychological - complications
- Stress, Psychological - physiopathology
- Find related publications in this database (Keywords)
- Animal model
- Necrotizing enterocolitis
- Irritable bowel syndrome
- Inflammatory bowel disease
- Rodent
- Mouse
- Rat