Medizinische Universität Graz Austria/Österreich - Forschungsportal - Medical University of Graz
Gewählte Publikation:
Lüneberg, E; Müller, D; Steinmetz, I; Frosch, M.
Monoclonal antibody against species-specific epitope of Pseudomonas aeruginosa Hsp60 protein cross-reacts with Pseudomonas stutzeri and other Pseudomonas species.
FEMS Microbiol Lett. 1997; 154(1):131-137
Doi: 10.1016/S0378-1097(97)00313-3
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PubMed
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- Co-Autor*innen der Med Uni Graz
- Steinmetz Ivo
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- Abstract:
- In a previous study, we determined the epitope of the Pseudomonas aeruginosa Hsp60 heat shock protein which is recognized by the specific monoclonal antibody (MAb) 2528. Subsequent investigations revealed a weak cross-reactivity of MAb 2528 with P. stutzeri, P. alcaligenes, P. mendocina and P. pseudoalcaligenes. To elucidate the molecular structure for these cross-reactions, we cloned the P. stutzeri hsp60 gene in Escherichia coli and determined the nucleotide sequence of the gene. In addition, the hsp60 gene of further Pseudomonas species was amplified and sequenced and amino acid substitutions within the epitope recognized by MAb 2528 were determined. The decapeptide QADIEARVLQ is unique to the P. aeruginosa Hsp60 protein, and cross-reaction of MAb 2528 reflects the phylogenetic relationship of Pseudomonas species as P. aeruginosa and all four cross-reacting species constitute a DNA homology group within the rRNA group I of the family Pseudomonadaceae, which belong to the gamma-subclass of the Proteobacteria.
- Find related publications in this database (using NLM MeSH Indexing)
- Amino Acid Sequence -
- Antibodies, Monoclonal -
- Antibody Specificity -
- Bacterial Proteins - analysis
- Bacterial Proteins - genetics
- Bacterial Proteins - immunology
- Blotting, Western -
- Chaperonin 60 - analysis
- Chaperonin 60 - genetics
- Chaperonin 60 - immunology
- Cloning, Molecular -
- Cross Reactions -
- DNA, Bacterial - analysis
- Epitopes - analysis
- Epitopes - immunology
- Molecular Sequence Data -
- Pseudomonas aeruginosa - chemistry
- Pseudomonas aeruginosa - genetics
- Pseudomonas aeruginosa - immunology
- Sequence Analysis, DNA -
- Species Specificity -
- Find related publications in this database (Keywords)
- Pseudomonas
- heat shock protein
- hsp60 gene
- molecular cloning