Medizinische Universität Graz - Research portal
Selected Publication:
SHR Neuro Cancer Cardio Lipid Metab Microb
Schweighofer, N; Aigelsreiter, A; Trummer, O; Graf-Rechberger, M; Hacker, N; Kniepeiss, D; Wagner, D; Stiegler, P; Trummer, C; Pieber, T; Obermayer-Pietsch, B; Müller, H.
Direct comparison of regulators of calcification between bone and vessels in humans.
Bone. 2016; 88:31-38
Doi: 10.1016/j.bone.2016.04.016
Web of Science
PubMed
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FullText_MUG
- Leading authors Med Uni Graz
- Obermayer-Pietsch Barbara
- Schweighofer Natascha
- Co-authors Med Uni Graz
- Aigelsreiter Ariane
- Graf-Rechberger Martina
- Hacker Nicole
- Kniepeiss Daniela
- Müller Helmut
- Pieber Thomas
- Stiegler Philipp
- Trummer Christian
- Trummer Olivia
- Wagner Doris
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- Abstract:
- Calcification is not only physiologically present in bone but is a main pathophysiological process in vasculature, favouring cardiovascular diseases. Our aim was to investigate changes in the expression of calcification regulators during vascular calcification in bone and vasculature. Levels of gene expression of osteoprotegerin (OPG), receptor activator of NF-κB ligand (RANKL), osteopontin (OPN), matrix gla protein (MGP), bone sialoprotein (BSP), SMAD6, and runt-related transcription factor 2 (RUNX2) were determined in bone, aorta, and external iliac artery tissue samples of transplant donors. Histological stages of atherosclerosis (AS) in vessels are defined as "no changes", "intima thickening", or "intima calcification". Patients' bone samples were subgrouped accordingly. We demonstrate that in vessels BSP and OPN expression significantly increased during intima thickening and decreased during intima calcification, whereas the expression of regulators of calcification did not significantly change in bone during intima thickening and intima calcification. At the stage of intima thickening, MGP, OPG, and SMAD6 expression and at stage of intima calcification only MGP expression was lower in bone than in vessel. The expression of BSP and RANKL was regulated in opposite ways in bone and vessels, whereas the expression of MGP, OC, RUNX2, and OPN was regulated in a tissue-specific manner. Our study is the first direct comparison of gene expression changes during AS progression in bone and vessels. Our results indicate that changes in the expression of regulators of calcification in the vessel wall as well as in bone occur early in the calcification process, even prior to deposition of calcium/phosphate precipitation. Copyright © 2016. Published by Elsevier Inc.
- Find related publications in this database (using NLM MeSH Indexing)
- Atherosclerosis - genetics
- Atherosclerosis - pathology
- Blood Vessels - pathology
- Bone and Bones - metabolism
- Bone and Bones - pathology
- Calcinosis - genetics
- Calcinosis - pathology
- Female -
- Gene Expression Regulation -
- Humans -
- Iliac Artery - pathology
- Male -
- Middle Aged -
- Find related publications in this database (Keywords)
- Vascular calcification
- Atherosclerosis
- Regulators of calcification
- Gene expression