Medizinische Universität Graz - Research portal
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SHR Neuro Cancer Cardio Lipid Metab Microb
Weiß, S; Somitsch, W; Klymiuk, I; Trajanoski, S; Guebitz, GM.
Comparison of biogas sludge and raw crop material as source of hydrolytic cultures for anaerobic digestion.
Bioresour Technol. 2016; 207(6):244-251
Doi: 10.1016/j.biortech.2016.01.137
Web of Science
PubMed
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FullText_MUG
- Co-authors Med Uni Graz
- Klymiuk Ingeborg
- Trajanoski Slave
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- Abstract:
- Mixed fermentative/hydrolytic bacteria were enriched on lignocellulose substrates in minimal medium under semi-anaerobic mesophilic conditions in the presence or absence of natural zeolite as growth supporter to ultimately bioaugment non-adapted sludge and thereby enhance the overall anaerobic digestion (AD) of recalcitrant plant material. Desired enzyme activities, i.e. xylanases and cellulase were monitored during subsequent cultivation cycles. Furthermore, enriched microbial communities were characterized by 16S rRNA-based 454-Pyrosequencing, revealing Firmicutes, Bacteriodetes, Proteobacteria and Spirochaetes to be the predominant bacterial groups in cultures derived from anaerobic sludge and raw crop material, i.e. maple green cut and wheat straw as well. Enriched populations relevant for biopolymer hydrolysis were then compared in biological methane potential tests to demonstrate positive effects on the biogasification of renewable plant substrate material. A significant impact on methane productivity was observed with adapted mixed cultures when used in combination with clinoptilolite to augment and supplement non-adapted bioreactor sludge. Copyright © 2016. Published by Elsevier Ltd.
- Find related publications in this database (using NLM MeSH Indexing)
- Anaerobiosis -
- Bacteria - genetics
- Bacteria - metabolism
- Biodegradation, Environmental -
- Biofuels -
- Cellulase - metabolism
- Crops, Agricultural - metabolism
- Hydrolases - metabolism
- Hydrolysis -
- Methane - biosynthesis
- RNA, Ribosomal, 16S -
- Refuse Disposal - methods
- Sewage - microbiology
- Find related publications in this database (Keywords)
- Bioaugmentation
- Biogas
- Green cut
- Wheat straw
- Zeolite