Medizinische Universität Graz Austria/Österreich - Forschungsportal - Medical University of Graz
Gewählte Publikation:
SHR Neuro Krebs Kardio Lipid Stoffw Microb
Pelzmann, B; Di Giuro, CM; Zorn-Pauly, K; Rossmann, C; Hallström, S; Groschner, K; Fameli, N.
Functional impairment of endothelial cells by the antimycotic amphotericin B.
Biochem Biophys Res Commun. 2016; 472(1):40-45
Doi: 10.1016/j.bbrc.2016.02.054
Web of Science
PubMed
FullText
FullText_MUG
- Führende Autor*innen der Med Uni Graz
- Fameli Nicola
- Pelzmann Brigitte
- Co-Autor*innen der Med Uni Graz
- Di Giuro Cristiana Maria Loredana
- Groschner Klaus
- Hallström Seth
- Rossmann Christine Renate
- Zorn-Pauly Klaus
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- Abstract:
- We set out to determine the membrane potential (Vm) of the endothelial cell line EA.hy926 and its sensitivity to the antimycotic amphotericin B (AmB), a commonly used antifungal component in cell culture media. We measured the endothelial Vm under various experimental conditions by patch clamp technique and found that Vm of AmB-treated cells is (-12.1 ± 9.3) mV, while in AmB-untreated (control) cells it is (-57.1 ± 4.1) mV. In AmB-free extracellular solutions, Vm recovered toward control levels and this gain in Vm rapidly dissipated upon re-addition of AmB, demonstrating a rapid and reversible effect of AmB on endothelial Vm. The consequences of AmB dependent alterations in endothelial transmembrane potential were tested at the levels of Ca(2+) signaling, of nucleotide concentrations, and energy metabolism. In AmB-treated cells we found substantially reduced Ca(2+) entry (to about 60% of that in control cells) in response to histamine induced endoplasmic reticulum (ER) Ca(2+) depletion, and diminished the ATP-to-ADP ratio (by >30%). Our data demonstrate a marked and experimentally relevant dependence of basic functional parameters of cultured endothelial cells on the presence of the ionophoric antimycotic AmB. The profound and reversible effects of the widely used culture media component AmB need careful consideration when interpreting experimental data obtained under respective culture conditions. Copyright © 2016 Elsevier Inc. All rights reserved.
- Find related publications in this database (using NLM MeSH Indexing)
- Adenosine Diphosphate - metabolism
- Adenosine Triphosphate - metabolism
- Amphotericin B - toxicity
- Antifungal Agents - toxicity
- Calcium Signaling - drug effects
- Cell Culture Techniques - methods
- Cell Line -
- Culture Media - toxicity
- Endothelial Cells - drug effects
- Endothelial Cells - metabolism
- Humans -
- Membrane Potentials - drug effects
- Patch-Clamp Techniques -
- Find related publications in this database (Keywords)
- Endothelium
- EA.hy926
- Membrane potential
- Calcium signals
- Depolarization
- Metabolism