Medizinische Universität Graz Austria/Österreich - Forschungsportal - Medical University of Graz
Gewählte Publikation:
Rabl, H; Ratschek, M; Khoschsorur, G; Uranüs, S; Tatzber, F; Tillian, HM; Esterbauer, H.
Limitation of Intestinal Reperfusion Injury in the Rat by Vitamin Treatment
MED SCI RES 1997 25: 315-318.
Web of Science
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- Co-Autor*innen der Med Uni Graz
- Khoschsorur Gholamali
- Ratschek Manfred
- Uranüs Selman
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- Abstract:
- The objective of the study was to evaluate the antioxidative effect of the multivitamin cocktail Omnibionta containing alpha-tocopherol, ascorbic acid, retinol and the vitamin B-complex, in an ischaemic-reperfusion model in the rat small bowel to reduce reperfusion injury. Animals were subjected to total superior mesenteric artery (SMA) occlusion for 40 min followed by reperfusion for 60 min. 67 Sprague Dawley rats were divided into the following groups : sham (n = 7), ischaemia (n = 12), reperfusion + saline (n = 12) reperfusion + omnibionta (n = 12), reperfusion + B-complex (n = 12), reperfusion + solvent (n = 12). The length of the variable damaged gut segments in % of total bowel length was calculated and termed the longitudinal damage (LD). Mucosal damage (MD) was assessed histologically using a 0 to 4 scoring scale similar to Chiu's method. Lipid peroxidation products were assayed by malondialdehyde-thiobarbituric acid reactive substances (MDA-TBARS). Reperfusion led to a significant (p < 0.05) increase in the length of the most severe damaged segment-seg 2-in the reperfused saline, solvent and B-complex group as compared to the ischaemic group (61.3 +/- 30.9%; 68.9 +/- 25.3%; 59.9 +/- 39.2%; vs. 6.3 +/- 8.4%). No difference existed between the reperfused Omnibionta (18.2 +/- 20.8%) and the ischaemic group (6.3 +/- 8.4%). Mucosal damage was significantly (p < 0.05) less severe in the reperfused Omnibionta group (3.3 +/- 3.8) than in all the other reperfused groups (normal saline 10.7 +/- 1.9; solvent 8.3 +/- 3.1; B-complex 7.2 +/- 4.8) and did not differ from the ischaemic group (4.6 +/- 5.9). A positive correlation (r = 0.361, p < 0.01) of MDA-TBARS existed with the histologically most severe damaged segment-seg 2-as a measure of free radical involvement 60 min after reperfusion onset. These results suggest that the multivitamin treatment may limit reperfusion damage in the rat intestine.
- Find related publications in this database (Keywords)
- Rat Intestine
- Ischemia-Reperfusion-Injury
- Mucosal Damage
- Longitudinal Damage
- Vitamin E
- Vitamin C and Vitamin a