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SHR Neuro Cancer Cardio Lipid Metab Microb
Lehmann, M; Gottschalk, B; Puchkov, D; Schmieder, P; Schwagerus, S; Hackenberger, CP; Haucke, V; Schmoranzer, J.
Multicolor Caged dSTORM Resolves the Ultrastructure of Synaptic Vesicles in the Brain.
Angew Chem Int Ed Engl. 2015; 54(45):13230-13235
Doi: 10.1002/anie.201505138
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- Leading authors Med Uni Graz
- Gottschalk Benjamin
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- Abstract:
- The precision of single-molecule localization-based super-resolution microscopy, including dSTORM, critically depends on the number of detected photons per localization. Recently, reductive caging of fluorescent dyes followed by UV-induced recovery in oxidative buffer systems was used to increase the photon yield and thereby the localization precision in single-color dSTORM. By screening 39 dyes for their fluorescence caging and recovery kinetics, we identify novel dyes that are suitable for multicolor caged dSTORM. Using a dye pair suited for registration error-free multicolor dSTORM based on spectral demixing (SD), a multicolor localization precision below 15 nm was achieved. Caged SD-dSTORM can resolve the ultrastructure of single 40 nm synaptic vesicles in brain sections similar to images obtained by immuno-electron microscopy, yet with much improved label density in two independent channels. © 2015 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.
- Find related publications in this database (using NLM MeSH Indexing)
- Animals -
- Brain - cytology
- Brain - ultrastructure
- Color -
- Fluorescent Dyes - chemistry
- Image Processing, Computer-Assisted -
- Mice -
- Microscopy, Fluorescence - methods
- Molecular Structure -
- NIH 3T3 Cells -
- Synaptic Vesicles - ultrastructure
- Find related publications in this database (Keywords)
- electron microscopy
- fluorescent probes
- immunochemistry
- NMR spectroscopy
- single-molecule studies