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Selected Publication:
SHR Neuro Cancer Cardio Lipid Metab Microb
Donnerer, J; Liebmann, I.
ERK1/2 Phosphorylation in the Rat Supraoptic Nucleus, Dorsal Raphe Nucleus, and Locus Coeruleus Neurons Following Noxious Stimulation to the Hind Paw.
Pharmacology. 2016; 97(1-2):57-62
Doi: 10.1159/000442211
Web of Science
PubMed
FullText
FullText_MUG
- Leading authors Med Uni Graz
- Donnerer Josef
- Co-authors Med Uni Graz
- Liebmann Ingrid
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- Abstract:
- Phospho-ERK1/2 (pERK1/2) fluorescence-immunohistochemistry is specifically well suited to mirror neuronal activity in the pain pathway at the cellular level. This study employed this method to visualize neuronal activity in 3 rat CNS nuclei following an acute noxious stimulation. The rat hind paw was stimulated either by heat or by a sequence of mustard oil and heat. Two min after the thermal stimulation or after the combined mustard oil and thermal stimulation, there was a significant increase in cells showing pERK1/2 immunoreactivity in the supraoptic nucleus (SON), in the dorsal raphe nucleus (DRN), and in the locus coeruleus (LC). Pretreatment with the opioid analgesic morphine or the N-methyl-D-aspartate antagonist MK-801 markedly attenuated ERK1/2 phosphorylation. These findings support the concept that the SON, the DRN, and the LC are integrated into pain processing at the hypothalamic and brain stem level. © 2015 S. Karger AG, Basel.
- Find related publications in this database (using NLM MeSH Indexing)
- Analgesics, Opioid - pharmacology
- Animals -
- Brain - physiopathology
- Dizocilpine Maleate - pharmacology
- Dorsal Raphe Nucleus - physiopathology
- Hot Temperature -
- Immunohistochemistry -
- Locus Coeruleus - physiopathology
- Pain - physiopathology
- Phosphorylation -
- Rats -
- Rats, Sprague-Dawley -
- Supraoptic Nucleus - physiopathology
- Find related publications in this database (Keywords)
- ERK1/2 phosphorylation
- Nociception
- Hypothalamus
- Brain stem nuclei
- Opioid analgesic