Medizinische Universität Graz Austria/Österreich - Forschungsportal - Medical University of Graz
Gewählte Publikation:
SHR Neuro Krebs Kardio Lipid Stoffw Microb
Lichtenegger, S; Bina, I; Roier, S; Bauernfeind, S; Keidel, K; Schild, S; Anthony, M; Reidl, J.
Characterization of lactate utilization and its implication on the physiology of Haemophilus influenzae.
Int J Med Microbiol. 2014; 304(3-4): 490-498.
Doi: 10.1016/j.ijmm.2014.02.010
[OPEN ACCESS]
Web of Science
PubMed
FullText
FullText_MUG
- Führende Autor*innen der Med Uni Graz
- Wagner-Lichtenegger Sabine
- Co-Autor*innen der Med Uni Graz
- Strießnig-Bina Isabelle
- Altmetrics:
- Dimensions Citations:
- Plum Analytics:
- Scite (citation analytics):
- Abstract:
- Haemophilus influenzae is a Gram-negative bacillus and a frequent commensal of the human nasopharynx. Earlier work demonstrated that in H. influenzae type b, l-lactate metabolism is associated with serum resistance and in vivo survival of the organism. To further gain insight into lactate utilization of the non-typeable (NTHi) isolate 2019 and laboratory prototype strain Rd KW20, deletion mutants of the l-lactate dehydrogenase (lctD) and permease (lctP) were generated and characterized. It is shown, that the apparent KM of l-lactate uptake is 20.1μM as determined for strain Rd KW20. Comparison of the COPD isolate NTHi 2019-R with the corresponding lctP knockout strain for survival in human serum revealed no lactate dependent serum resistance. In contrast, we observed a 4-fold attenuation of the mutant strain in a murine model of nasopharyngeal colonization. Characterization of lctP transcriptional control shows that the lactate utilization system in H. influenzae is not an inductor inducible system. Rather negative feedback regulation was observed in the presence of l-lactate and this is dependent on the ArcAB regulatory system. Additionally, for 2019 it was found that lactate may have signaling function leading to increased cell growth in late log phase under conditions where no l-lactate is metabolized. This effect seems to be ArcA independent and was not observed in strain Rd KW20. We conclude that l-lactate is an important carbon-source and may act as host specific signal substrate which fine tunes the globally acting ArcAB regulon and may additionally affect a yet unknown signaling system and thus may contribute to enhanced in vivo survival. Copyright © 2014 Elsevier GmbH. All rights reserved.
- Find related publications in this database (using NLM MeSH Indexing)
- Animals -
- Blood Bactericidal Activity -
- Disease Models, Animal -
- Energy Metabolism -
- Gene Deletion -
- Gene Expression Profiling -
- Gene Expression Regulation, Bacterial -
- Haemophilus Infections - microbiology
- Haemophilus influenzae - genetics Haemophilus influenzae - metabolism Haemophilus influenzae - physiology
- L-Lactate Dehydrogenase - genetics L-Lactate Dehydrogenase - metabolism
- Lactic Acid - metabolism
- Membrane Transport Proteins - genetics Membrane Transport Proteins - metabolism
- Mice, Inbred BALB C -
- Microbial Viability -
- Nasopharynx - microbiology
- Serum - microbiology
- Virulence -
- Find related publications in this database (Keywords)
- L-Lactate utilization
- lctP
- Haemophilus influenzae
- Colonization