Medizinische Universität Graz - Research portal
Selected Publication:
SHR Neuro Cancer Cardio Lipid Metab Microb
Kleinhans, C; Mohan, RR; Vacun, G; Schwarz, T; Haller, B; Sun, Y; Kahlig, A; Kluger, P; Finne-Wistrand, A; Walles, H; Hansmann, J.
A perfusion bioreactor system efficiently generates cell-loaded bone substitute materials for addressing critical size bone defects.
Biotechnol J. 2015; 10(11):1727-1738
Doi: 10.1002/biot.201400813
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- Leading authors Med Uni Graz
- Kleinhans Claudia
- Co-authors Med Uni Graz
- Schwarz Thomas
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- Abstract:
- Critical size bone defects and non-union fractions are still challenging to treat. Cell-loaded bone substitutes have shown improved bone ingrowth and bone formation. However, a lack of methods for homogenously colonizing scaffolds limits the maximum volume of bone grafts. Additionally, therapy robustness is impaired by heterogeneous cell populations after graft generation. Our aim was to establish a technology for generating grafts with a size of 10.5 mm in diameter and 25 mm of height, and thus for grafts suited for treatment of critical size bone defects. Therefore, a novel tailor-made bioreactor system was developed, allowing standardized flow conditions in a porous poly(L-lactide-co-caprolactone) material. Scaffolds were seeded with primary human mesenchymal stem cells derived from four different donors. In contrast to static experimental conditions, homogenous cell distributions were accomplished under dynamic culture. Additionally, culture in the bioreactor system allowed the induction of osteogenic lineage commitment after one week of culture without addition of soluble factors. This was demonstrated by quantitative analysis of calcification and gene expression markers related to osteogenic lineage. In conclusion, the novel bioreactor technology allows efficient and standardized conditions for generating bone substitutes that are suitable for the treatment of critical size defects in humans. © 2015 The Authors. Biotechnology Journal published by Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim. This is an open access article under the terms of the Creative Commons Attribution Non-Commercial Licence, which permits use, distribution and reproduction in any medium, provided the Contribution is properly cited and is not used for commercial purpose.
- Find related publications in this database (using NLM MeSH Indexing)
- Bioreactors -
- Bone Substitutes - metabolism
- Cell Survival -
- Cells, Cultured -
- Humans -
- Materials Testing -
- Mesenchymal Stem Cells - cytology
- Perfusion - methods
- Polyesters -
- Tissue Engineering - methods
- Tissue Scaffolds -
- Find related publications in this database (Keywords)
- Bone substitute
- Critical size defect
- Perfusion bioreactor system
- Poly(LLA-co-CL) scaffold
- Tissue engineering