Medizinische Universität Graz Austria/Österreich - Forschungsportal - Medical University of Graz
Gewählte Publikation:
SHR Neuro Krebs Kardio Lipid Stoffw Microb
Hasenoehrl, C; Alexander, CM; Azzarelli, NN; Dabrowiak, JC.
Enhanced detection of gold nanoparticles in agarose gel electrophoresis.
Electrophoresis. 2012; 33(8): 1251-1254.
Doi: 10.1002/elps.201100556
Web of Science
PubMed
FullText
FullText_MUG
- Führende Autor*innen der Med Uni Graz
- Hasenöhrl Carina
- Altmetrics:
- Dimensions Citations:
- Plum Analytics:
- Scite (citation analytics):
- Abstract:
- Gel electrophoresis is a powerful tool in gold nanoparticle (AuNP) research. While the technique is sensitive to the size, charge, and shape of particles, its optimal performance requires a relatively large amount of AuNP in the loading wells for visible detection of bands. We here describe a novel and more sensitive method for detecting AuNPs in agarose gels that involves staining the gel with the common organic fluorophore fluorescein, to produce AuNP band intensities that are linear with nanoparticle concentration and almost an order of magnitude larger than those obtained without staining the gel. © 2012 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.
- Find related publications in this database (using NLM MeSH Indexing)
- Electrophoresis, Agar Gel - methods
- Fluorescein - chemistry
- Fluorescent Dyes - chemistry
- Gold - analysis Gold - chemistry
- Metal Nanoparticles - analysis Metal Nanoparticles - chemistry
- Particle Size -
- Sensitivity and Specificity -
- Find related publications in this database (Keywords)
- Background staining
- Gel electrophoresis
- Gold nanoparticles