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Selected Publication:
SHR Neuro Cancer Cardio Lipid Metab Microb
Knabl, J; Hiden, U; Hüttenbrenner, R; Riedel, C; Hutter, S; Kirn, V; Günthner-Biller, M; Desoye, G; Kainer, F; Jeschke, U.
GDM Alters Expression of Placental Estrogen Receptor α in a Cell Type and Gender-Specific Manner.
Reprod Sci. 2015; 22(12):1488-1495
Doi: 10.1177/1933719115585147
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- Leading authors Med Uni Graz
- Hiden Ursula
- Co-authors Med Uni Graz
- Desoye Gernot
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- Abstract:
- The nuclear receptor estrogen receptor α (ERα) is one of the key players in energy balance, insulin resistance, and trophoblast differentiation. We tested the hypothesis that gestational diabetes mellitus (GDM) alters expression of placental ERα in a cell type-specific manner and that this regulation may involve epigenetic changes. Expression of ERα was analyzed by immunohistochemistry using the semiquantitative immunoreactive score in 80 placentas (40 GDM/40 controls). Quantitative real-time polymerase chain reaction (PCR) measured ERα messenger RNA (mRNA) in decidual tissue. Methylation-specific PCR was performed to analyze cytosine-phosphatidyl-guanine-island methylation of the ERα promoter. Expression of ERα protein is upregulated (P = .011) in GDM in extravillous trophoblasts but not in syncytiotrophoblast. Gestational diabetes mellitus downregulated ERα in decidual vessels only in pregnancies with male but not female fetuses. Furthermore, mRNA of the ERα encoding gene estrogen receptor gene 1 (ESR1) was increased (+1.77 fold) in GDM decidua when compared to controls (P = .024). In parallel, the promoter of ESR1 was methylated only in decidua of healthy control individuals but not in GDM. Gestational diabetes mellitus affects expression of placental ERα in a cell type-dependent way, on epigenetic level. These data link GDM with epigenetic deregulations of ERα expression and open new insights into the intrauterine programming hypothesis of GDM. © The Author(s) 2015.
- Find related publications in this database (using NLM MeSH Indexing)
- Adult -
- Blood Vessels - metabolism
- Blood Vessels - pathology
- Case-Control Studies -
- CpG Islands -
- DNA Methylation -
- Diabetes, Gestational - genetics
- Diabetes, Gestational - metabolism
- Diabetes, Gestational - pathology
- Down-Regulation -
- Epigenesis, Genetic -
- Estrogen Receptor alpha - genetics
- Estrogen Receptor alpha - metabolism
- Female -
- Fetus - metabolism
- Gene Expression Regulation, Developmental -
- Gestational Age -
- Humans -
- Immunohistochemistry -
- Male -
- Placenta - blood supply
- Placenta - metabolism
- Placenta - pathology
- Pregnancy -
- Promoter Regions, Genetic -
- RNA, Messenger - genetics
- RNA, Messenger - metabolism
- Real-Time Polymerase Chain Reaction -
- Reverse Transcriptase Polymerase Chain Reaction -
- Sex Determination Processes -
- Sex Factors -
- Trophoblasts - metabolism
- Trophoblasts - pathology
- Find related publications in this database (Keywords)
- gestational diabetes
- estrogen
- nuclear receptor
- methylation
- trophoblast