Medizinische Universität Graz Austria/Österreich - Forschungsportal - Medical University of Graz
Gewählte Publikation:
SHR Neuro Krebs Kardio Lipid Stoffw Microb
Weinberger, J; Jimenez-Heredia, R; Schaller, S; Suessner, S; Sunzenauer, J; Reindl-Schwaighofer, R; Weiss, R; Winkler, S; Gabriel, C; Danzer, M; Oberbauer, R.
Immune Repertoire Profiling Reveals that Clonally Expanded B and T Cells Infiltrating Diseased Human Kidneys Can Also Be Tracked in Blood.
PLoS One. 2015; 10(11):e0143125-e0143125
Doi: 10.1371/journal.pone.0143125
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- Co-Autor*innen der Med Uni Graz
- GABRIEL Christian
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- Abstract:
- Recent advances in high-throughput sequencing allow for the competitive analysis of the human B and T cell immune repertoire. In this study we compared Immunoglobulin and T cell receptor repertoires of lymphocytes found in kidney and blood samples of 10 patients with various renal diseases based on next-generation sequencing data. We used Biomed-2 primer panels and ImmunExplorer software to sequence, analyze and compare complementarity determining regions and V-(D)-J elements. While generally an individual's renal receptor repertoire is different from the repertoire present in blood, 94% (30/32) of the lymphocytes with clonal expansion in kidney can also be traced in blood however, not all of these clonotypes are equally abundant. Summarizing the data of all analyzed patients, 68% of highly expanded T cell clonotypes and 30% of the highly expanded B cell clonotypes that have infiltrated the kidney can be found amongst the five most abundant clonotypes in blood. In addition, complementarity determining region 3 sequences of the immunoglobulin heavy chains are on average more diverse than T cell receptor beta chains. Immune repertoire analysis of tissue infiltrating B and T cells adds new approaches to the assessment of adaptive immune response in kidney diseases. Our data suggest that expanded clonotypes in the tissues might be traceable in blood samples in the course of treatment or the natural history of the disease.
- Find related publications in this database (using NLM MeSH Indexing)
- Adult -
- Aged -
- Aged, 80 and over -
- Amino Acid Sequence -
- B-Lymphocytes - immunology
- Cell Proliferation -
- Clone Cells -
- Complementarity Determining Regions - chemistry
- Genetic Variation -
- Humans -
- Kidney - immunology
- Kidney Diseases - blood
- Middle Aged -
- Molecular Sequence Data -
- T-Lymphocytes - immunology
- VDJ Exons - genetics
- Young Adult -