Medizinische Universität Graz Austria/Österreich - Forschungsportal - Medical University of Graz
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SHR Neuro Krebs Kardio Lipid Stoffw Microb
Russ, M; Hauser, S; Wintersteiger, R; Greilberger, J; Andrä, M; Ortner, A.
Determination of Angiotensin-(1-7) with HPLC/Fluorescence-Detection.
J Fluoresc. 2016; 26(1):81-6
Doi: 10.1007/s10895-015-1686-y
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- Co-Autor*innen der Med Uni Graz
- Andrä Michaela
- Greilberger Joachim
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- Abstract:
- Angiotensin-(1-7) is an important active component in the renin-angiotensin-system. Due to its cardio protective effects it is now under investigation in combination with antioxidants as a reperfusion solution. The combination showed impressive effects on isolated hearts of male Wistar rats after induced ischemia. In this work a high performance liquid chromatography method with fluorescence detection was developed for the first time for in-process measurements as well as for stability tests of the peptide in the novel antioxidant-containing Karal® solution. For fluorescence detection of angiotensin-(1-7) fluorescamine as derivatization dye was applied. Under optimized conditions the method showed linearity over the range of 50 to 5000 ng/mL with R(2) of 0.9988 and an overall precision better than 5.0 %. LOD and LOQ were determined to be in the femtomol range on column. It was found that stability of angiotensin-(1-7) could be significantly improved in the antioxidant containing preparation compared to aqueous solutions.
- Find related publications in this database (using NLM MeSH Indexing)
- Angiotensin I - analysis
- Animals - administration & dosage
- Chromatography, High Pressure Liquid - administration & dosage
- Fluorescence - administration & dosage
- Male - administration & dosage
- Microscopy, Fluorescence - administration & dosage
- Molecular Conformation - administration & dosage
- Peptide Fragments - analysis
- Rats - administration & dosage
- Rats, Wistar - administration & dosage
- Find related publications in this database (Keywords)
- Angiotensin-(1-7)
- Fluorescamine
- HPLC
- Fluorescence detection
- Stability