Medizinische Universität Graz Austria/Österreich - Forschungsportal - Medical University of Graz
Gewählte Publikation:
SHR Neuro Krebs Kardio Lipid Stoffw Microb
Lohberger, B; Kaltenegger, H; Stuendl, N; Rinner, B; Leithner, A; Sadoghi, P.
Impact of cyclic mechanical stimulation on the expression of extracellular matrix proteins in human primary rotator cuff fibroblasts.
Knee Surg Sports Traumatol Arthrosc. 2016; 24(12):3884-3891
Doi: 10.1007/s00167-015-3790-6
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- Führende Autor*innen der Med Uni Graz
- Lohberger Birgit
- Co-Autor*innen der Med Uni Graz
- Eck Nicole
- Kaltenegger Heike
- Leithner Andreas
- Rinner Beate
- Sadoghi Patrick
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- Abstract:
- Mechanical stimulation plays an important role in the development and remodelling of tendons. The aim of the study was to evaluate the effects of mechanical stimulation on the expression of extracellular matrix proteins in human primary rotator cuff (RC) fibroblasts. RC fibroblasts were isolated from patients with degenerative RC tears and characterized using flow cytometry and immunohistochemistry. Cells were stimulated using the Flexcell FX5K™ Tension System. The stimulation regime was a uniaxial sinusoidal waveform with 10 % elongation and a frequency of 0.5 Hz, whereby each cycle consists of 10-s strain and 30-s relaxation. Data were normalized to mechanically unstimulated control groups for every experimental condition. RT-qPCR was performed to determine relative mRNA levels, and collagen production was measured by a colorimetric assay. The positive expression of CD91 and CD10, and negativity for CD45 and CD4 confirmed the fibroblast phenotype of RC primary cells. RT-qPCR revealed that 10 % continuous cyclic strain for 7 and 14 days induced a significant increase in the mRNA expression both on the matrix metalloproteinases MMP1, MMP3, MMP13, and MMP14 and on the extracellular matrix proteins decorin, tenascin-C, and scleraxis. Furthermore, mechanically stimulated groups produced significantly higher amounts of total collagen. These results may contribute to a better understanding of strain-induced tendon remodelling and will form the basis for the correct choice of applied force in rehabilitation after orthopaedic surgery. These findings underline the fact that early passive motion of the joint in order to induce remodelling of the tendon should be included within a rehabilitation protocol for rotator cuff repair.
- Find related publications in this database (using NLM MeSH Indexing)
- Aged -
- Basic Helix-Loop-Helix Transcription Factors - genetics
- Basic Helix-Loop-Helix Transcription Factors - metabolism
- Collagen - genetics
- Collagen - metabolism
- Extracellular Matrix Proteins - genetics
- Extracellular Matrix Proteins - metabolism
- Female -
- Fibroblasts - metabolism
- Humans -
- Male -
- Matrix Metalloproteinase 1 - metabolism
- Matrix Metalloproteinase 13 - genetics
- Matrix Metalloproteinase 13 - metabolism
- Matrix Metalloproteinase 14 - genetics
- Matrix Metalloproteinase 14 - metabolism
- Matrix Metalloproteinase 3 - metabolism
- Middle Aged -
- Orthopedic Procedures -
- Physical Stimulation - methods
- RNA, Messenger - metabolism
- Real-Time Polymerase Chain Reaction -
- Rotator Cuff - cytology
- Rotator Cuff Injuries -
- Tenascin - genetics
- Tenascin - metabolism
- Tendons - metabolism
- Find related publications in this database (Keywords)
- Rotator cuff fibroblasts
- Mechanical stimulation
- Collagen
- Extracellular matrix proteins