Medizinische Universität Graz - Research portal
Selected Publication:
SHR Neuro Cancer Cardio Lipid Metab Microb
Kroneis, T; El-Heliebi, A.
Whole Genome Amplification of Labeled Viable Single Cells Suited for Array-Comparative Genomic Hybridization.
Methods Mol Biol. 2015; 1347(22):233-243
Doi: 10.1007/978-1-4939-2990-0_16
PubMed
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- Leading authors Med Uni Graz
- Kroneis Thomas
- Co-authors Med Uni Graz
- El-Heliebi Amin
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- Abstract:
- Understanding details of a complex biological system makes it necessary to dismantle it down to its components. Immunostaining techniques allow identification of several distinct cell types thereby giving an inside view of intercellular heterogeneity. Often staining reveals that the most remarkable cells are the rarest. To further characterize the target cells on a molecular level, single cell techniques are necessary. Here, we describe the immunostaining, micromanipulation, and whole genome amplification of single cells for the purpose of genomic characterization. First, we exemplify the preparation of cell suspensions from cultured cells as well as the isolation of peripheral mononucleated cells from blood. The target cell population is then subjected to immunostaining. After cytocentrifugation target cells are isolated by micromanipulation and forwarded to whole genome amplification. For whole genome amplification, we use GenomePlex(®) technology allowing downstream genomic analysis such as array-comparative genomic hybridization.
- Find related publications in this database (using NLM MeSH Indexing)
- Comparative Genomic Hybridization - methods
- DNA Fragmentation -
- Genome, Human -
- Genomic Library -
- Genomics - methods
- Humans -
- Immunohistochemistry -
- Leukocytes, Mononuclear - metabolism
- Nucleic Acid Amplification Techniques -
- Polymerase Chain Reaction -
- Single-Cell Analysis - methods