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Selected Publication:
SHR Neuro Cancer Cardio Lipid Metab Microb
El-Heliebi, A; Chen, S; Kroneis, T.
Using Multiplex PCR for Assessing the Quality of Whole Genome Amplified DNA.
Methods Mol Biol. 2015; 1347(22):119-128
Doi: 10.1007/978-1-4939-2990-0_9
PubMed
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- Leading authors Med Uni Graz
- El-Heliebi Amin
- Kroneis Thomas
- Co-authors Med Uni Graz
- Chen Shukun
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- Abstract:
- This chapter describes a simple and inexpensive multiplex PCR-based method to assess the quality of whole genome amplification (WGA) products generated from heat-induced random fragmented DNA. A set of four primer pairs is used to amplify DNA sequences of WGA products in and downstream of GAPDH gene in yielding 100, 200, 300, and 400 bp fragments. PCR products are analyzed by agarose gel electrophoresis and the respective WGA quality is classified according to the number of obtained PCR bands. WGA products that yield three or four PCR bands are considered to be of high quality and yield good results when analyzed by means of array comparative genome hybridization (CGH).
- Find related publications in this database (using NLM MeSH Indexing)
- DNA -
- Genome -
- Genomics - methods
- Genomics - standards
- Multiplex Polymerase Chain Reaction - methods
- Nucleic Acid Amplification Techniques -
- Quality Control -
- Single-Cell Analysis - methods
- Single-Cell Analysis - standards