Medizinische Universität Graz - Research portal
Selected Publication:
SHR Neuro Cancer Cardio Lipid Metab Microb
Kroneis, T; El-Heliebi, A.
Whole Genome Amplification by Isothermal Multiple Strand Displacement Using Phi29 DNA Polymerase.
Methods Mol Biol. 2015; 1347(22):111-117
Doi: 10.1007/978-1-4939-2990-0_8
PubMed
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- Leading authors Med Uni Graz
- Kroneis Thomas
- Co-authors Med Uni Graz
- El-Heliebi Amin
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- Abstract:
- The here described method of isothermal whole genome amplification (iWGA) uses a Phi29 DNA polymerase-based kit (Illustra GenomiPhi V2 DNA Amplification Kit) that amplifies minute quantities of DNA by multiple strand displacement upon random hexamer primer binding. Starting from genomic DNA or single cells this amplification yields up to 5 μg of iWGA product with fragment lengths of 10 kb and longer. As this amplification lacks the need of fragmenting DNA, its products are well suited for many downstream applications (e.g. sequencing and DNA profiling). On the contrary, degraded DNA samples are not supported by the nature of the amplification and are not well suited.
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