Medizinische Universität Graz - Research portal
Selected Publication:
SHR Neuro Cancer Cardio Lipid Metab Microb
El-Heliebi, A; Chen, S; Kroneis, T.
Heat-Induced Fragmentation and Adapter-Assisted Whole Genome Amplification Using GenomePlex® Single-Cell Whole Genome Amplification Kit (WGA4).
Methods Mol Biol. 2015; 1347(22):101-109
Doi: 10.1007/978-1-4939-2990-0_7
PubMed
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- Leading authors Med Uni Graz
- El-Heliebi Amin
- Kroneis Thomas
- Co-authors Med Uni Graz
- Chen Shukun
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- Abstract:
- Whole genome amplification (WGA) is a widely used technique allowing multiplying picogram amounts of target DNA by several orders of magnitude. The technique described here is based on heat-induced random fragmentation yielding DNA strands mainly ranging from 0.1 to 1 kb in length. The fragmented DNA is then subjected to library generation by annealing of adaptor sequences to both ends of the DNA fragments. Using primers hybridizing to the adapter sequences, the DNA is amplified by thermal cycling. This amplification typically yields > 2 mg DNA from a single cell, is suited for amplifying DNA isolated from (partly) degraded samples [e.g. formalin-fixed paraffin-embedded (FFPE) material] and works well when used for array-comparative genome hybridization (array-CGH).
- Find related publications in this database (using NLM MeSH Indexing)
- DNA Fragmentation -
- Genome -
- Genomics - methods
- Nucleic Acid Amplification Techniques -
- Reagent Kits, Diagnostic -
- Single-Cell Analysis - methods