Medizinische Universität Graz Austria/Österreich - Forschungsportal - Medical University of Graz
Gewählte Publikation:
SHR Neuro Krebs Kardio Lipid Stoffw Microb
Bagdonaite, I; Wandall, HH; Litvinov, IV; Nastasi, C; Becker, JC; Dabelsteen, S; Geisler, C; Bonefeld, CM; Zhang, Q; Wasik, MA; Zhou, Y; Sasseville, D; Ødum, N; Woetmann, A.
Ectopic expression of a novel CD22 splice-variant regulates survival and proliferation in malignant T cells from cutaneous T cell lymphoma (CTCL) patients.
Oncotarget. 2015; 6(16):14374-14384
Doi: 10.18632/oncotarget.3720
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- Co-Autor*innen der Med Uni Graz
- Becker Jürgen Christian
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- Abstract:
- CD22 is a member of the Sialic acid-binding Ig-like lectin (Siglec) family of lectins described to be exclusively present in B lymphocytes and B cell-derived neoplasms. Here, we describe a novel splice form of CD22 (designated CD22âN), which lacks the N-terminal domain as demonstrated by exon-specific RT-PCR and differential recognition by anti-CD22 antibodies. Importantly, CD22âN mRNA is expressed in skin lesions from 39 out of 60 patients with cutaneous T cell lymphoma (CTCL), whereas few patients (6 out of 60) expresses full-length, wild type CD22 (CD22wt). In addition, IHC staining of tumor biopsies confirmed the expression of CD22 in CD4+ T cells. Moreover, four out of four malignant T cell lines express CD22: Two cell lines express CD22âN (MyLa2059 and PB2B) and two express CD22wt (MAC-1 and MAC-2A). siRNA-mediated silencing of CD22 impairs proliferation and survival of malignant T cells, demonstrating a functional role for both CD22âN and CD22wt in these cells.In conclusion, we provide the first evidence for an ectopic expression of CD22 and a novel splice variant regulating malignant proliferation and survival in CTCL. Analysis of expression and function of CD22 in cutaneous lymphomas may form the basis for development of novel targeted therapies for our patients.
- Find related publications in this database (using NLM MeSH Indexing)
- Animals -
- Cell Line, Tumor -
- Humans -
- Lymphocyte Activation -
- Lymphoma, T-Cell, Cutaneous - genetics
- Lymphoma, T-Cell, Cutaneous - immunology
- Lymphoma, T-Cell, Cutaneous - metabolism
- Mice -
- Protein Isoforms -
- RNA Splicing -
- Sialic Acid Binding Ig-like Lectin 2 - biosynthesis
- Sialic Acid Binding Ig-like Lectin 2 - genetics
- T-Lymphocytes - immunology
- T-Lymphocytes - metabolism
- Transfection -
- Find related publications in this database (Keywords)
- CTCL
- CD22