Medizinische Universität Graz - Research portal
Selected Publication:
SHR Neuro Cancer Cardio Lipid Metab Microb
Surmeneva, MA; Kleinhans, C; Vacun, G; Kluger, PJ; Schönhaar, V; Müller, M; Hein, SB; Wittmar, A; Ulbricht, M; Prymak, O; Oehr, C; Surmenev, RA.
Nano-hydroxyapatite-coated metal-ceramic composite of iron-tricalcium phosphate: Improving the surface wettability, adhesion and proliferation of mesenchymal stem cells in vitro.
Colloids Surf B Biointerfaces. 2015; 135(10):386-393
Doi: 10.1016/j.colsurfb.2015.07.057
Web of Science
PubMed
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- Co-authors Med Uni Graz
- Kleinhans Claudia
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- Abstract:
- Thin radio-frequency magnetron sputter deposited nano-hydroxyapatite (HA) films were prepared on the surface of a Fe-tricalcium phosphate (Fe-TCP) bioceramic composite, which was obtained using a conventional powder injection moulding technique. The obtained nano-hydroxyapatite coated Fe-TCP biocomposites (nano-HA-Fe-TCP) were studied with respect to their chemical and phase composition, surface morphology, water contact angle, surface free energy and hysteresis. The deposition process resulted in a homogeneous, single-phase HA coating. The ability of the surface to support adhesion and the proliferation of human mesenchymal stem cells (hMSCs) was studied using biological short-term tests in vitro. The surface of the uncoated Fe-TCP bioceramic composite showed an initial cell attachment after 24h of seeding, but adhesion, proliferation and growth did not persist during 14 days of culture. However, the HA-Fe-TCP surfaces allowed cell adhesion, and proliferation during 14 days. The deposition of the nano-HA films on the Fe-TCP surface resulted in higher surface energy, improved hydrophilicity and biocompatibility compared with the surface of the uncoated Fe-TCP. Furthermore, it is suggested that an increase in the polar component of the surface energy was responsible for the enhanced cell adhesion and proliferation in the case of the nano-HA-Fe-TCP biocomposites. Copyright © 2015 Elsevier B.V. All rights reserved.
- Find related publications in this database (using NLM MeSH Indexing)
- Alkaline Phosphatase - analysis
- Alkaline Phosphatase - metabolism
- Calcium Phosphates - chemistry
- Cell Adhesion -
- Cell Differentiation - drug effects
- Cell Proliferation - drug effects
- Ceramics - chemistry
- Durapatite - chemistry
- Humans -
- Iron - chemistry
- Mesenchymal Stem Cells - drug effects
- Mesenchymal Stem Cells - ultrastructure
- Metal Nanoparticles - chemistry
- Surface Properties -
- Find related publications in this database (Keywords)
- Bioceramic composite
- Bioresorbable alloy
- Hydroxyapatite coating
- RF magnetron sputtering
- Cell adhesion