Medizinische Universität Graz Austria/Österreich - Forschungsportal - Medical University of Graz
Gewählte Publikation:
SHR Neuro Krebs Kardio Lipid Stoffw Microb
Bode, GH; Pickl, KE; Sanchez-Purrà, M; Albaiges, B; Borrós, S; Pötgens, AJ; Schmitz, C; Sinner, FM; Losen, M; Steinbusch, HW; Frank, HG; Martinez-Martinez, P; European NanoBioPharmaceutics Research Initiative.
Detection of Peptide-based nanoparticles in blood plasma by ELISA.
PLoS One. 2015; 10(5):e0126136-e0126136
Doi: 10.1371/journal.pone.0126136
[OPEN ACCESS]
Web of Science
PubMed
FullText
FullText_MUG
- Co-Autor*innen der Med Uni Graz
- Sinner Frank Michael
- Altmetrics:
- Dimensions Citations:
- Plum Analytics:
- Scite (citation analytics):
- Abstract:
- The aim of the current study was to develop a method to detect peptide-linked nanoparticles in blood plasma. A convenient enzyme linked immunosorbent assay (ELISA) was developed for the detection of peptides functionalized with biotin and fluorescein groups. As a proof of principle, polymerized pentafluorophenyl methacrylate nanoparticles linked to biotin-carboxyfluorescein labeled peptides were intravenously injected in Wistar rats. Serial blood plasma samples were analyzed by ELISA and by liquid chromatography mass spectrometry (LC/MS) technology. The ELISA based method for the detection of FITC labeled peptides had a detection limit of 1 ng/mL. We were able to accurately measure peptides bound to pentafluorophenyl methacrylate nanoparticles in blood plasma of rats, and similar results were obtained by LC/MS. We detected FITC-labeled peptides on pentafluorophenyl methacrylate nanoparticles after injection in vivo. This method can be extended to detect nanoparticles with different chemical compositions.
- Find related publications in this database (using NLM MeSH Indexing)
- Animals -
- Chromatography, Liquid -
- Enzyme-Linked Immunosorbent Assay - methods
- Male -
- Nanoparticles -
- Peptides - blood
- Plasma -
- Rats -
- Tandem Mass Spectrometry -