Medizinische Universität Graz Austria/Österreich - Forschungsportal - Medical University of Graz
Gewählte Publikation:
SHR Neuro Krebs Kardio Lipid Stoffw Microb
Rabensteiner, J; Theiler, G; Duettmann, W; Zollner-Schwetz, I; Hoenigl, M; Valentin, T; Leitner, E; Luxner, J; Grisold, A; Valentin, A; Neumeister, P; Krause, R.
Detection of central venous catheter-related bloodstream infections in haematooncological patients.
Eur J Clin Invest. 2015; 45(8):824-832
Doi: 10.1111/eci.12477
Web of Science
PubMed
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- Führende Autor*innen der Med Uni Graz
- Krause Robert
- Rabensteiner Jasmin
- Co-Autor*innen der Med Uni Graz
- Düttmann Wiebke
- Grisold Andrea
- Hönigl Martin
- Leitner-Meyer Eva
- Luxner Josefa
- Neumeister Peter
- Theiler Georg
- Valentin Angelika
- Valentin Thomas
- Zollner-Schwetz Ines
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- Abstract:
- Catheter-related bloodstream infections (CRBSIs) are currently detected in patients with clinically suspicion. The aim of our study was to evaluate whether CRBSIs could be anticipated and detected in a subclinical stage by peptide nucleic acid fluorescence in situ hybridization (PNA FISH) using universal hybridization probes or acridine orange leucocyte cytospin (AOLC) tests in haematooncological patients with central venous catheters (CVCs) in situ. Peptide nucleic acid fluorescence in situ hybridization and AOLC tests using blood samples from one CVC lumen/port chamber in haematooncological patients were continuously performed. These results were compared to those obtained from routinely performed CRBSI diagnostic tests. One hundred and eighty-two patients with 342 catheter periods were investigated. Seventeen CRBSI cases were detected in 6466 CVC days by routine measures resulting in a CRBSI rate of 2.6/1000 catheter days. Two of 17 showed positive PNA FISH tests, and five positive AOLC test results before the diagnosis were established with routine measures. The screening revealed further seven patients with positive universal PNA FISH tests and 10 positive AOLC tests without symptoms indicative for infection and were therefore considered not to have CRBSI. Sampling of only one CVC lumen/port chamber screening for CRBSI in haematooncological patients seems not to be a useful tool for anticipative diagnosis of CRBSI. Reasons for false-negative results might include origin of CRBSIs from the other CVC lumina not sampled for screening, and false-positive results might origin from catheter colonization without subsequent spread of micro-organisms into the peripheral bloodstream. © 2015 Stichting European Society for Clinical Investigation Journal Foundation.
- Find related publications in this database (using NLM MeSH Indexing)
- Acridine Orange -
- Adult -
- Aged -
- Bacteremia - complications
- Bacteremia - diagnosis
- Candidiasis - complications
- Candidiasis - diagnosis
- Catheter-Related Infections - complications
- Catheter-Related Infections - diagnosis
- Central Venous Catheters -
- Cohort Studies -
- Enterobacteriaceae Infections - complications
- Enterobacteriaceae Infections - diagnosis
- Escherichia coli Infections - complications
- Escherichia coli Infections - diagnosis
- False Negative Reactions -
- Female -
- Fluorescent Dyes -
- Fungemia - complications
- Fungemia - diagnosis
- Gram-Negative Bacterial Infections - complications
- Gram-Negative Bacterial Infections - diagnosis
- Gram-Positive Bacterial Infections - complications
- Gram-Positive Bacterial Infections - diagnosis
- Hematologic Neoplasms - complications
- Humans -
- In Situ Hybridization, Fluorescence -
- Klebsiella Infections - complications
- Klebsiella Infections - diagnosis
- Male -
- Middle Aged -
- Peptide Nucleic Acids -
- Pseudomonas Infections - complications
- Pseudomonas Infections - diagnosis
- Staphylococcal Infections - complications
- Staphylococcal Infections - diagnosis
- Stenotrophomonas maltophilia -
- Find related publications in this database (Keywords)
- Acridine orange leucocyte cytospin
- catheter-related blood stream infection
- haematooncology
- peptide nucleic acid fluorescence insitu hybridization
- screening