Medizinische Universität Graz Austria/Österreich - Forschungsportal - Medical University of Graz
Gewählte Publikation:
SHR Neuro Krebs Kardio Lipid Stoffw Microb
Störmer, M; Arroyo, A; Brachert, J; Carrero, H; Devine, D; Epstein, JS; Gabriel, C; Gelber, C; Goodrich, R; Hanschmann, KM; Heath, DG; Jacobs, MR; Keil, S; de Korte, D; Lambrecht, B; Lee, CK; Marcelis, J; Marschner, S; McDonald, C; McGuane, S; McKee, M; Müller, TH; Muthivhi, T; Pettersson, A; Radziwon, P; Ramirez-Arcos, S; Reesink, HW; Rojo, J; Rood, I; Schmidt, M; Schneider, CK; Seifried, E; Sicker, U; Wendel, S; Wood, EM; Yomtovian, RA; Montag, T.
Establishment of the first international repository for transfusion-relevant bacteria reference strains: ISBT working party transfusion-transmitted infectious diseases (WP-TTID), subgroup on bacteria.
Vox Sang. 2012; 102(1): 22-31.
Doi: 10.1111/j.1423-0410.2011.01510.x
Web of Science
PubMed
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FullText_MUG
- Co-Autor*innen der Med Uni Graz
- GABRIEL Christian
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- Abstract:
- Bacterial contamination of platelet concentrates (PCs) still remains a significant problem in transfusion with potential important clinical consequences, including death. The International Society of Blood Transfusion Working Party on Transfusion-Transmitted Infectious Diseases, Subgroup on Bacteria, organised an international study on Transfusion-Relevant Bacteria References to be used as a tool for development, validation and comparison of both bacterial screening and pathogen reduction methods. Four Bacteria References (Staphylococcus epidermidis PEI-B-06, Streptococcus pyogenes PEI-B-20, Klebsiella pneumoniae PEI-B-08 and Escherichia coli PEI-B-19) were selected regarding their ability to proliferate to high counts in PCs and distributed anonymised to 14 laboratories in 10 countries for identification, enumeration and bacterial proliferation in PCs after low spiking (0·3 and 0·03 CFU/ml), to simulate contamination occurring during blood donation. Bacteria References were correctly identified in 98% of all 52 identifications. S. pyogenes and E. coli grew in PCs in 11 out of 12 laboratories, and K. pneumoniae and S. epidermidis replicated in all participating laboratories. The results of bacterial counts were very consistent between laboratories: the 95% confidence intervals were for S. epidermidis: 1·19-1·32 × 10(7) CFU/ml, S. pyogenes: 0·58-0·69 × 10(7) CFU/ml, K. pneumoniae: 18·71-20·26 × 10(7) CFU/ml and E. coli: 1·78-2·10 × 10(7) CFU/ml. The study was undertaken as a proof of principle with the aim to demonstrate (i) the quality, stability and suitability of the bacterial strains for low-titre spiking of blood components, (ii) the property of donor-independent proliferation in PCs, and (iii) their suitability for worldwide shipping of deep frozen, blinded pathogenic bacteria. These aims were successfully fulfilled. The WHO Expert Committee Biological Standardisation has approved the adoption of these four bacteria strains as the first Repository for Transfusion-Relevant Bacteria Reference Strains and, additionally, endorsed as a project the addition of six further bacteria strain preparations suitable for control of platelet contamination as the next step of enlargement of the repository. © 2011 The Author(s). Vox Sanguinis © 2011 International Society of Blood Transfusion.
- Find related publications in this database (using NLM MeSH Indexing)
- Bacterial Infections - prevention & control
- Bacterial Typing Techniques - methods
- Bacteriological Techniques -
- Biological Specimen Banks -
- Blood Component Transfusion - methods
- Blood Platelets - cytology Blood Platelets - microbiology
- Blood Transfusion -
- Escherichia coli - metabolism
- Humans -
- International Cooperation -
- Klebsiella pneumoniae - metabolism
- Quality Assurance, Health Care - methods
- Reproducibility of Results -
- Staphylococcus epidermidis - metabolism
- Streptococcus pyogenes - metabolism
- Find related publications in this database (Keywords)
- bacteria reference strains
- bacterial contamination
- international reference material
- pathogen reduction
- platelet screening
- repository
- validation