Medizinische Universität Graz Austria/Österreich - Forschungsportal - Medical University of Graz
Gewählte Publikation:
SHR Neuro Krebs Kardio Lipid Stoffw Microb
Danzer, M; Polin, H; Pröll, J; Hofer, K; Fae, I; Fischer, GF; Gabriel, C.
High-throughput sequence-based typing strategy for HLA-DRB1 based on real-time polymerase chain reaction.
Hum Immunol. 2007; 68(11): 915-917.
Doi: 10.1016/j.humimm.2007.10.005
Web of Science
PubMed
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- Co-Autor*innen der Med Uni Graz
- GABRIEL Christian
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- Abstract:
- DNA sequencing is the gold standard for high-resolution genotyping of the highly polymorphic human leukocyte antigen (HLA) loci. In the case of hematopoietic stem cell transplantation, four-digit typing of HLA class I and II genes is indicated. We developed a group-specific, real-time polymerase chain reaction (PCR) strategy for amplification of DRB1 applying TaqMan chemistry on different real-time machines. For evaluation of genotyping accuracy and ambiguity resolution, 115 well-characterized samples were adapted. Separate analysis of each allele was possible in 100 samples (87%). Of the samples, 13% (n = 15) showed amplification in one of the eight group-specific PCR mixes: one sample according to the group DRB1*15, 16, along with 14 samples according to the group DRB1*03, *11, *13, *14. Further testing of the 14 (12.2%) samples associated with group DRB1*03, *11, *13, *14 was performed with specific intron primers. In conclusion this typing scheme generates results within 1 day, thereby making sequencing for different requirements attractive.
- Find related publications in this database (using NLM MeSH Indexing)
- Alleles -
- Base Sequence -
- HLA-DR Antigens - genetics
- HLA-DRB1 Chains -
- Humans -
- Polymerase Chain Reaction - methods
- Reproducibility of Results -
- Sensitivity and Specificity -
- Find related publications in this database (Keywords)
- HLA-DRB1
- SBT
- real-time PCR
- TaqMan