Medizinische Universität Graz Austria/Österreich - Forschungsportal - Medical University of Graz
Gewählte Publikation:
SHR Neuro Krebs Kardio Lipid Stoffw Microb
Wolbank, S; Peterbauer, A; Fahrner, M; Hennerbichler, S; van Griensven, M; Stadler, G; Redl, H; Gabriel, C.
Dose-dependent immunomodulatory effect of human stem cells from amniotic membrane: a comparison with human mesenchymal stem cells from adipose tissue.
Tissue Eng. 2007; 13(6): 1173-1183.
Doi: 10.1089/ten.2006.0313
Web of Science
PubMed
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- Co-Autor*innen der Med Uni Graz
- GABRIEL Christian
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- Abstract:
- Bone marrow-derived mesenchymal stem cells (BMSCs) have been used for allogeneic application in tissue engineering but have certain drawbacks. Therefore, stem cells (SC)s derived from other adult tissue sources have been considered as an alternative. However, there is only limited knowledge on their immunomodulatory properties. The aim of our study was to compare the immunomodulatory potential of human amniotic mesenchymal and human amniotic epithelial cells with that of human adipose-derived SCs under identical experimental conditions. We have demonstrated a dose-dependent inhibition of peripheral blood mononuclear cell (PBMC) immune responses in mixed lymphocyte reactions (up to 66-93% inhibition) and phytohemagglutinin activation assays (up to 67-96% inhibition). The lowest SC-to-PBMC ratio able to inhibit PBMC proliferation significantly was 1:8. Subcultivation (passage 2-6) did not alter immunoinhibitory properties, whereas cryopreservation significantly reduced the immunomodulatory potential. Using transwell systems, we have demonstrated an inhibition mechanism that is dependent on cell contact. Additionally, in coculture with allogeneic PBMCs, SCs were well tolerated and at most provoked mild alloreactions in singular cases. This study demonstrates, for the first time, contact- and dose-dependent immunosuppression of mesenchymal and epithelial amniotic SC populations, as well as of adipose tissue-derived SCs. All three cell types may be considered as possible alternatives to BMSCs for allogeneic application in tissue engineering.
- Find related publications in this database (using NLM MeSH Indexing)
- Adipocytes - cytology Adipocytes - immunology
- Amnion - cytology Amnion - immunology
- Cell Communication - immunology
- Cell Differentiation -
- Cells, Cultured -
- Dose-Response Relationship, Drug -
- Humans -
- Immunologic Factors - immunology
- Lymphocyte Activation - immunology
- Lymphocytes - immunology
- Mesenchymal Stromal Cells - cytology Mesenchymal Stromal Cells - immunology
- Stem Cells - cytology Stem Cells - immunology
- Tissue Engineering - methods