Medizinische Universität Graz Austria/Österreich - Forschungsportal - Medical University of Graz
Gewählte Publikation:
SHR Neuro Krebs Kardio Lipid Stoffw Microb
Kemptner, J; Witzeneder, K; Hennerbichler, S; Gabriel, C.
Proteomic analysis of human-derived cell culture supplements.
Transfus Med Hemother. 2013; 40(6):413-415
Doi: 10.1159/000356241
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- Führende Autor*innen der Med Uni Graz
- GABRIEL Christian
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- Abstract:
- Development of cell therapy and advanced therapy medicinal products depends on in vitro expansion of human cells in fetal bovine serum (FBS) supplemented media. Human-derived supplements, such as human serum (huS) and human platelet lysate (hPL), represent suitable alternatives to FBS. Various studies demonstrated that the use of these human alternatives result in comparable or even improved proliferation and expansion ratios. Within this study three human supplement alternatives, huS, hPLP (plasma containing hPL) and hPLN (plasma replaced by saline), were compared by 2D gel electrophoresis, an important tool in proteomic analysis. 2D gel electrophoresis allows the determination of the protein number and the detection of protein changes (decreasing/increasing concentration). The comparison of huS, hPLP, and hPLN gels resulted in clearly visible differences in protein pattern, protein number and concentration, particularly when comparing huS with hPL and hPLP with hPLN.
- Find related publications in this database (Keywords)
- Cell therapy
- Human platelet lysate
- Human serum
- Fetal calf serum
- Proteomics