Medizinische Universität Graz Austria/Österreich - Forschungsportal - Medical University of Graz
Gewählte Publikation:
SHR Neuro Krebs Kardio Lipid Stoffw Microb
Hager, G; Holnthoner, W; Wolbank, S; Husa, AM; Godthardt, K; Redl, H; Gabriel, C.
Three specific antigens to isolate endothelial progenitor cells from human liposuction material.
Cytotherapy. 2013; 15(11): 1426-1435.
Doi: 10.1016/j.jcyt.2013.06.018
Web of Science
PubMed
FullText
FullText_MUG
- Co-Autor*innen der Med Uni Graz
- GABRIEL Christian
- Altmetrics:
- Dimensions Citations:
- Plum Analytics:
- Scite (citation analytics):
- Abstract:
- Human endothelial progenitor cells (EPC) play an important role in regenerative medicine and contribute to neovascularization on vessel injury. They are usually enriched from peripheral blood, cord blood and bone marrow. In human fat tissue, EPC are rare and their isolation remains a challenge. Fat tissue was prepared by collagenase digestion, and the expression of specific marker proteins was evaluated by flow cytometry in the stromal vascular fraction (SVF). For enrichment, magnetic cell sorting was performed with the use of CD133 microbeads and EPC were cultured until colonies appeared. A second purification was performed with CD34; additional isolation steps were performed with the use of a combination of CD34 and CD31 microbeads. Enriched cells were investigated by flow cytometry for the expression of endothelial specific markers, by Matrigel assay and by the uptake of acetylated low-density lipoprotein. The expression pattern confirmed the heterogeneous nature of the SVF, with rare numbers of CD133+ detectable. EPC gained from the SVF by magnetic enrichment showed cobblestone morphology of outgrowth endothelial cells and expressed the specific markers CD31, CD144, vascular endothelial growth factor (VEGF)R2, CD146, CD73 and CD105. Functional integrity was confirmed by uptake of acetylated low-density lipoprotein and the formation of tube-like structures on Matrigel. Rare EPC can be enriched from human fat tissue by magnetic cell sorting with the use of a combination of microbeads directed against CD133, an early EPC marker, CD34, a stem cell marker, and CD31, a typical marker for endothelial cells. In culture, they differentiate into EC and hence could have the potential to contribute to neovascularization in regenerative medicine. Copyright © 2013 International Society for Cellular Therapy. Published by Elsevier Inc. All rights reserved.
- Find related publications in this database (using NLM MeSH Indexing)
- 5'-Nucleotidase - biosynthesis
- Adipose Tissue - cytology
- Antigens, CD - biosynthesis Antigens, CD - immunology
- Antigens, CD146 - biosynthesis
- Antigens, CD31 - biosynthesis Antigens, CD31 - immunology
- Antigens, CD34 - immunology
- Cadherins - biosynthesis
- Cell Differentiation - immunology
- Cells, Cultured -
- Endothelial Cells - cytology Endothelial Cells - immunology
- GPI-Linked Proteins - biosynthesis
- Glycoproteins - immunology
- Humans -
- Lipectomy -
- Microspheres -
- Neovascularization, Physiologic - physiology
- Peptides - immunology
- Receptors, Cell Surface - biosynthesis
- Stem Cells - cytology Stem Cells - immunology
- Vascular Endothelial Growth Factor Receptor-2 - biosynthesis
- Find related publications in this database (Keywords)
- endothelial progenitor cells
- liposuction
- magnetic cell sorting
- vascularization