Medizinische Universität Graz Austria/Österreich - Forschungsportal - Medical University of Graz
Gewählte Publikation:
SHR Neuro Krebs Kardio Lipid Stoffw Microb
Gabriel, C; Fürst, D; Faé, I; Wenda, S; Zollikofer, C; Mytilineos, J; Fischer, GF.
HLA typing by next-generation sequencing - getting closer to reality.
Tissue Antigens. 2014; 83(2): 65-75.
Doi: 10.1111/tan.12298
Web of Science
PubMed
FullText
FullText_MUG
- Co-Autor*innen der Med Uni Graz
- GABRIEL Christian
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- Abstract:
- Next generation sequencing (NGS) denotes novel sequencing technologies that enable the generation of a large number of clonal sequences in a single sequencing run. NGS was initially introduced for whole genome sequencing and for quantitation of viral variants or genetic mutations in tumor tissues; more recently, the potential for high resolution HLA typing and high throughput analyses has been explored. It became clear that the complexity of the HLA system implicates new challenges, especially for bioinformatics. From an economical point of view, NGS is becoming increasingly attractive for HLA typing laboratories currently relying on Sanger based sequencing. Realizing the full potential of NGS will require the development of specifically adapted typing strategies and software algorithms. In the present review, three laboratories that were among the first to perform HLA-typing using different NGS platforms, the Roche 454, the Illumina Miseq and the Ion Torrent system, respectively, give an overview of these applications and point out advantages and limitations. © 2014 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd.
- Find related publications in this database (using NLM MeSH Indexing)
- Algorithms -
- HLA Antigens - classification HLA Antigens - genetics
- High-Throughput Nucleotide Sequencing - instrumentation High-Throughput Nucleotide Sequencing - methods High-Throughput Nucleotide Sequencing - standards
- Histocompatibility Testing - instrumentation Histocompatibility Testing - methods Histocompatibility Testing - standards
- Humans -
- Research Design -
- Sequence Analysis, DNA -
- Software -
- Find related publications in this database (Keywords)
- 454 Roche
- ambiguities
- human leukocyte antigen typing
- Illumina
- Ion Torrent
- next-generation sequencing
- sequencing platforms