Medizinische Universität Graz Austria/Österreich - Forschungsportal - Medical University of Graz
Gewählte Publikation:
SHR Neuro Krebs Kardio Lipid Stoffw Microb
Lindenmair, A; Wolbank, S; Stadler, G; Meinl, A; Peterbauer-Scherb, A; Eibl, J; Polin, H; Gabriel, C; van Griensven, M; Redl, H.
Osteogenic differentiation of intact human amniotic membrane.
Biomaterials. 2010; 31(33): 8659-8665.
Doi: 10.1016/j.biomaterials.2010.07.090
Web of Science
PubMed
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FullText_MUG
- Co-Autor*innen der Med Uni Graz
- GABRIEL Christian
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- Abstract:
- Tissue engineering strategies usually require cell isolation and combination with a suitable biomaterial. Human amniotic membrane (AM) represents a natural two-layered sheet comprising cells with proven stem cell characteristics. In our approach, we evaluated the differentiation potential of AM in toto with its sessile stem cells as alternative to conventional approaches requiring cell isolation and combination with biomaterials. For this, AM-biopsies were differentiated in vitro using two osteogenic media compared with control medium (CM) for 28 days. Mineralization and osteocalcin expression was demonstrated by (immuno)histochemistry. Alkaline phosphatase (AP) activity, calcium contents and mRNA expression of RUNX2, AP, osteopontin, osteocalcin, BMP-2 (bone morphogenetic protein), and BMP-4 were quantified and AM viability was evaluated. Under osteogenic conditions, AM-biopsies mineralized successfully and by day 28 the majority of cells expressed osteocalcin. This was confirmed by a significant rise in calcium contents (up to 27.4 ± 6.8 mg/dl d28), increased AP activity, and induction of RUNX2, AP, BMP-2 and BMP-4 mRNA expression. Relatively high levels of viability were retained, especially in osteogenic media (up to 78.3 ± 19.0% d14; 62.9 ± 22.3% d28) compared to CM (42.2 ± 15.2% d14; 35.1 ± 8.6% d28). By this strategy, stem cells within human AM can successfully be driven along the osteogenic pathways while residing within their natural environment. Copyright © 2010 Elsevier Ltd. All rights reserved.
- Find related publications in this database (using NLM MeSH Indexing)
- Alkaline Phosphatase - metabolism
- Amnion - cytology Amnion - drug effects Amnion - enzymology
- Biological Markers - metabolism
- Calcium - metabolism
- Cell Differentiation - drug effects
- Cell Survival - drug effects
- Cells, Cultured -
- Culture Media - pharmacology
- Gene Expression Regulation - drug effects
- Humans -
- Intracellular Space - drug effects Intracellular Space - enzymology
- Osteogenesis - drug effects
- Reverse Transcriptase Polymerase Chain Reaction -
- Find related publications in this database (Keywords)
- Human amniotic membrane
- Sessile stem cells
- Biomineralization
- Bone tissue engineering
- Osteogenesis