Medizinische Universität Graz Austria/Österreich - Forschungsportal - Medical University of Graz
Gewählte Publikation:
SHR Neuro Krebs Kardio Lipid Stoffw Microb
Niklas, N; Pröll, J; Weinberger, J; Zopf, A; Wiesinger, K; Krismer, K; Bettelheim, P; Gabriel, C.
Qualifying high-throughput immune repertoire sequencing.
Cell Immunol. 2014; 288(1-2): 31-38.
Doi: 10.1016/j.cellimm.2014.02.001
Web of Science
PubMed
FullText
FullText_MUG
- Co-Autor*innen der Med Uni Graz
- GABRIEL Christian
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- Abstract:
- Diversity of B and T cell receptors, achieved by gene recombination and somatic hypermutation, allows the immune system for recognition and targeted reaction against various threats. Next-generation sequencing for assessment of a cell's gene composition and variation makes deep analysis of one individual's immune spectrum feasible. An easy to apply but detailed analysis and visualization strategy is necessary to process all sequences generated. We performed sequencing utilizing the 454 system for CLL and control samples, utilized the IMGT database and applied the presented analysis tools. With the applied protocol, malignant clones are found and characterized, mutational status compared to germline identity is elaborated in detail showing that the CLL mutation status is not as monoclonal as generally thought. On the other hand, this strategy is not solely applicable to the 454 sequencing system but can easily be transferred to any other next-generation sequencing platform. Copyright © 2014 Elsevier Inc. All rights reserved.
- Find related publications in this database (using NLM MeSH Indexing)
- Base Sequence -
- Case-Control Studies -
- Clone Cells -
- Genome, Human -
- Germ-Line Mutation -
- High-Throughput Nucleotide Sequencing - standards
- Humans -
- Leukemia, Lymphocytic, Chronic, B-Cell - genetics Leukemia, Lymphocytic, Chronic, B-Cell - immunology Leukemia, Lymphocytic, Chronic, B-Cell - pathology
- Molecular Sequence Data -
- Phylogeny -
- Receptors, Antigen, B-Cell - classification Receptors, Antigen, B-Cell - genetics Receptors, Antigen, B-Cell - immunology
- Receptors, Antigen, T-Cell - classification Receptors, Antigen, T-Cell - genetics Receptors, Antigen, T-Cell - immunology
- Sequence Alignment -
- Sequence Homology, Nucleic Acid -
- Find related publications in this database (Keywords)
- Next-generation sequencing
- Immune repertoire
- Clonotyping
- Immunoglobulin heavy chain
- T cell receptor p
- VDJ recombination
- Diversity generation
- Somatic hypermutation
- Big data visualization