Medizinische Universität Graz Austria/Österreich - Forschungsportal - Medical University of Graz
Gewählte Publikation:
SHR Neuro Krebs Kardio Lipid Stoffw Microb
Hofmann, AD; Friedmacher, F; Takahashi, T; Gosemann, JH; Puri, P.
Increased pulmonary vascular expression of receptor for advanced glycation end products (RAGE) in experimental congenital diaphragmatic hernia.
J Pediatr Surg. 2015; 50(5):746-749
Doi: 10.1016/j.jpedsurg.2015.02.024
Web of Science
PubMed
FullText
FullText_MUG
- Co-Autor*innen der Med Uni Graz
- Friedmacher Florian
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- Abstract:
- Persistent pulmonary hypertension (PH) continues to be a major cause of high mortality in congenital diaphragmatic hernia (CDH). The receptor for advanced glycation end products (RAGE) is a member of the immunoglobulin protein family. Recently, RAGE has been implicated in mediating pulmonary arterial smooth muscle cell proliferation and vascular remodeling in experimental PH. RAGE has been reported to be highly upregulated in lung tissue of patients with severe PH. We designed this study to investigate the hypothesis that RAGE expression is increased in nitrofen-induced CDH. Pregnant rats were exposed to nitrofen or vehicle on D9. Fetuses were sacrificed on D21 and divided into nitrofen (n=16) and control group (n=16). Quantitative real-time polymerase chain reaction, Western blotting, and confocal immunofluorescence were performed. Pulmonary RAGE gene expression levels were significantly increased in nitrofen-induced CDH compared to controls (p<0.003). Western blotting and confocal microscopy revealed increased pulmonary RAGE protein expression in CDH compared to controls. This study provides striking evidence of increased gene and protein expression of RAGE in the pulmonary vasculature of nitrofen-induced CDH, suggesting that increased expression of RAGE may play a role in the pathogenesis of PH in nitrofen-induced CDH. Copyright © 2015 Elsevier Inc. All rights reserved.
- Find related publications in this database (using NLM MeSH Indexing)
- Animals -
- Animals, Newborn -
- Blotting, Western -
- DNA - genetics
- Female -
- Gene Expression Regulation, Developmental -
- Hernia, Diaphragmatic - chemically induced
- Hernias, Diaphragmatic, Congenital - chemically induced
- Hernias, Diaphragmatic, Congenital - genetics
- Hernias, Diaphragmatic, Congenital - metabolism
- Lung - metabolism
- Pregnancy -
- Pregnancy, Animal -
- Rats -
- Rats, Sprague-Dawley -
- Real-Time Polymerase Chain Reaction -
- Receptor for Advanced Glycation End Products - biosynthesis
- Receptor for Advanced Glycation End Products - genetics
- Find related publications in this database (Keywords)
- CDH
- Nitrofen
- RAGE
- Pulmonary hypertension
- Vascular remodeling