Medizinische Universität Graz Austria/Österreich - Forschungsportal - Medical University of Graz
Gewählte Publikation:
SHR Neuro Krebs Kardio Lipid Stoffw Microb
Lindenmair, A; Nürnberger, S; Stadler, G; Meinl, A; Hackl, C; Eibl, J; Gabriel, C; Hennerbichler, S; Redl, H; Wolbank, S.
Intact human amniotic membrane differentiated towards the chondrogenic lineage.
Cell Tissue Bank. 2014; 15(2): 213-225.
Doi: 10.1007/s10561-014-9454-9
Web of Science
PubMed
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- Co-Autor*innen der Med Uni Graz
- GABRIEL Christian
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- Abstract:
- Human amniotic membrane (hAM) represents a tissue that is well established as biomaterial in the clinics with potential for new applications in regenerative medicine. For tissue engineering (TE) strategies, cells are usually combined with inductive factors and a carrier substrate. We have previously recognized that hAM represents a natural, preformed sheet including highly potent stem cells. In the present approach for cartilage regeneration we have induced chondrogenesis in hAM in vitro. For this, hAM biopsies were cultured for up to 56 days under chondrogenic conditions. The induced hAM was characterized for remaining viability, glycosaminoglycan (GAG) accumulation using histochemical analysis, and a quantitative assay. Collagen I, II and X was immunohistochemically determined and cartilage-specific mRNA expression of (sex determining region Y-) box 9, cartilage oligomeric matrix protein (COMP), aggrecan (AGC1), versican (CSPG2), COL1A1, COL9A2, melanoma inhibitory activity (MIA), and cartilage-linking protein 1 (CRTL1) analyzed by quantitative real-time polymerase chain reaction. Human AM was successfully induced to accumulate GAG, as demonstrated by Alcianblue staining and a significant (p < 0.001) increase of GAG/viability under chondrogenic conditions peaking in a 29.9 ± 0.9-fold induction on day 56. Further, upon chondrogenic induction collagen II positive areas were identified within histological sections and cartilage-specific markers including COMP, AGC1, CSPG2, COL1A1, COL9A2, MIA, and CRTL1 were found upregulated at mRNA level. This is the first study, demonstrating that upon in vitro induction viable human amnion expresses cartilage-specific markers and accumulates GAGs within the biomatrix. This is a promising first step towards a potential use of living hAM for cartilage TE.
- Find related publications in this database (using NLM MeSH Indexing)
- Amnion - cytology
- Cartilage - cytology
- Cell Differentiation - physiology
- Cell Lineage - physiology
- Cells, Cultured -
- Chondrogenesis - physiology
- Female -
- Humans -
- Placenta - cytology
- Pregnancy -
- Stem Cells - cytology
- Find related publications in this database (Keywords)
- Amniotic membrane
- Chondrogenic
- Differentiation
- Tissue engineering
- Cartilage
- Human