Medizinische Universität Graz Austria/Österreich - Forschungsportal - Medical University of Graz
Gewählte Publikation:
Formanek, M; Knerer, B; Temmel, A; Thurnher, D; Millesi, W; Kornfehl, J.
Oral keratinocytes derived from the peritonsillar mucosa express the proinflammatory cytokine IL-6 without prior stimulation.
J Oral Pathol Med. 1998; 27(5):202-206
Doi: 10.1111/j.1600-0714.1998.tb01942.x
Web of Science
PubMed
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- Co-Autor*innen der Med Uni Graz
- Thurnher Dietmar
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- Abstract:
- The aim of the present study is to examine the cytokine expression and corresponding receptor pattern of human oral mucosa-derived keratinocytes. The mRNA expression of these cytokines from isolated and purified cells was measured by a RT-PCR method, the protein production by ELISA, and the receptor expression was determined by FACS analysis. In freshly isolated oral keratinocytes, IL-1alpha, IL-1alpha receptor antagonist, IL-6, IL-8, TGF-beta, TNF-alpha, and bFGF were detectable at the protein and mRNA level, whereas PDGF and TGF-alpha were found only at the mRNA level. There were no detectable signals for IL-2 and IL-4. The cytokine production at the protein level was independent from stimulation with PMA (phorbol myristate acetate). Unstimulated commercially available and primary isolated epidermal keratinocytes showed similar cytokine pattern except a lack of IL-6. FACS analysis revealed receptor expression on oral keratinocytes for IL-1, IL-2, IL-4, IL-6, EGF, IFN-gamma, and PDGF. In addition, receptor mRNA for IL-8, TNF-alpha, FGF-2 and KGF could be detected, but not for IL-10. Our results show that human oral keratinocytes produce a cytokine panel comparable to epidermal keratinocytes. In contrast to the epidermis, IL-6 was produced by human oral keratinocytes constitutively without prior stimulation, which may indicate their active regulation role in the maintenance of the oral mucosa.
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- Cell Separation -
- Enzyme-Linked Immunosorbent Assay -
- Fibroblast Growth Factor 2 - genetics Fibroblast Growth Factor 2 - metabolism
- Flow Cytometry -
- Gene Expression -
- Humans -
- Interleukin-1 - genetics Interleukin-1 - metabolism
- Interleukin-2 - genetics Interleukin-2 - metabolism
- Interleukin-4 - genetics Interleukin-4 - metabolism
- Interleukin-6 - genetics Interleukin-6 - metabolism
- Interleukin-8 - genetics Interleukin-8 - metabolism
- Keratinocytes - metabolism
- Mouth Mucosa - cytology Mouth Mucosa - metabolism
- Palatine Tonsil - cytology Palatine Tonsil - metabolism
- Platelet-Derived Growth Factor - genetics Platelet-Derived Growth Factor - metabolism
- Polymerase Chain Reaction -
- RNA, Messenger - analysis RNA, Messenger - genetics
- Receptors, Interleukin - genetics Receptors, Interleukin - metabolism
- Receptors, Interleukin-1 - antagonists & inhibitors
- Receptors, Interleukin-6 - genetics Receptors, Interleukin-6 - metabolism
- Skin - cytology Skin - metabolism
- Tetradecanoylphorbol Acetate - pharmacology
- Transforming Growth Factor alpha - genetics Transforming Growth Factor alpha - metabolism
- Transforming Growth Factor beta - genetics Transforming Growth Factor beta - metabolism
- Tumor Necrosis Factor-alpha - genetics Tumor Necrosis Factor-alpha - metabolism
- Find related publications in this database (Keywords)
- activation
- cytokines
- keratinocytes
- oral