Medizinische Universität Graz - Research portal
Selected Publication:
Thurnher, D; Bakroeva, M; Schütz, G; Pelzmann, M; Formanek, M; Knerer, B; Kornfehl, J.
Non-steroidal anti-inflammatory drugs induce apoptosis in head and neck cancer cell lines.
Acta Otolaryngol. 2001; 121(8):957-962
Doi: 10.1080/000164801317166862
Web of Science
PubMed
FullText
FullText_MUG
- Leading authors Med Uni Graz
- Thurnher Dietmar
- Altmetrics:
- Dimensions Citations:
- Plum Analytics:
- Scite (citation analytics):
- Abstract:
- Non-steroidal anti-inflammatory drugs (NSAIDs) can inhibit tumorigenesis in colorectal cancer due to the induction of apoptosis. Disturbances of cellular pathways ultimately leading to apoptosis may contribute to the process of neoplastic transformation and immortalization. In this study we wanted to determine the influence of different NSAIDs (indomethacin, ibuprofen and sodium salicylate) and hydrocortisone on Bcl-2 expression and the apoptotic behavior of head and neck tumor cell lines and normal oral keratinocytes. Bcl-2 expression was determined by monoclonal antibody staining and fluorescence-activated cell-sorting measurement. Apoptotic cells were visualized with a epifluorescence microscope after staining with CytoDeath M30 antibody. Indomethacin (1 mM) and ibuprofen (1 mM) significantly reduced Bcl-2 expression in the cancer cell lines tested and might be thought responsible for the observed increase in apoptosis. At all concentrations tested the influence of sodium salicylate and hydrocortisone on Bcl-2 expression was not significant. In contrast, the NSAIDs tested had only a minor influence on normal oral keratinocytes. Our results demonstrate a significant reduction in growth and an increase in apoptosis, possibly due to a reduction in Bcl-2 expression. after exposure to indomethacin and ibuprofen in the head and neck cancer cell lines tested.
- Find related publications in this database (using NLM MeSH Indexing)
- Anti-Inflammatory Agents - pharmacology
- Anti-Inflammatory Agents, Non-Steroidal - pharmacology Anti-Inflammatory Agents, Non-Steroidal - therapeutic use
- Antibodies, Monoclonal - immunology
- Apoptosis - drug effects
- Carcinoma, Squamous Cell - drug therapy Carcinoma, Squamous Cell - immunology Carcinoma, Squamous Cell - pathology
- Cyclin D1 - drug effects Cyclin D1 - immunology
- Head and Neck Neoplasms - drug therapy Head and Neck Neoplasms - immunology Head and Neck Neoplasms - pathology
- Humans -
- Keratinocytes - drug effects
- Microscopy, Fluorescence - methods
- Mouth Mucosa - pathology
- Steroids -
- Tumor Cells, Cultured -
- Find related publications in this database (Keywords)
- apoptosis
- Bcl-2
- cancer
- non-steroidal anti-inflammatory drugs