Medizinische Universität Graz - Research portal

Go directly to the nagivation.
Go directly to the content.

Navigation/Search

Selected Publication:

SHR Neuro Cancer Cardio Lipid Metab Microb

Heiduschka, G; Erovic, BM; Vormittag, L; Skoda, C; Martinek, H; Brunner, M; Ehrenberger, K; Thurnher, D.
7beta-hydroxycholesterol induces apoptosis and regulates cyclooxygenase 2 in head and neck squamous cell carcinoma.
Arch Otolaryngol Head Neck Surg. 2009; 135(3):261-267 Doi: 10.1001/archoto.2008.558
Web of Science PubMed FullText FullText_MUG

Leading authors Med Uni Graz: Thurnher Dietmar
Altmetrics:
Dimensions Citations:
Plum Analytics:
Scite (citation analytics):
Abstract:: To determine whether treatment with 7beta-hydroxycholesterol (7beta-HC) would trigger cell death in head and neck squamous cell carcinoma (HNSCC) cell lines in a dose-dependent fashion. In vitro study. The study included HNSCC cell lines SCC9, SCC25, CAL27, and FaDu. We treated HNSCC cell lines with increasing doses of 7beta-HC. Proliferation assays were performed to assess cell viability after treatment. Western blots were carried out to evaluate cyclooxygenase (COX)-1 and -2 expression levels. Using proliferation assays and immunocytochemical analysis, we detected significant growth inhibition via apoptosis in 4 different HNSCC cell lines after treatment with 7beta-HC (P < .001). The 50% inhibitory concentration levels were between 13.19 and 20.79 micromol/L after 72 hours. Western analysis indicated that COX-2, but not COX-1, levels were suppressed after treatment. Treatment with 7beta-HC resulted in suppression of HNSCC growth in vitro. Our data warrant further investigations for the potential use of 7beta-HC as a cytotoxic agent in head and neck cancer.
Find related publications in this database (using NLM MeSH Indexing): Apoptosis - drug effects; Blotting, Western -; Carcinoma, Squamous Cell - drug therapy; Carcinoma, Squamous Cell - enzymology; Carcinoma, Squamous Cell - pathology; Cell Count -; Cell Line, Tumor -; Cell Proliferation - drug effects; Cyclooxygenase 2 - drug effects; Cyclooxygenase 2 - metabolism; Dose-Response Relationship, Drug -; Flow Cytometry -; Head and Neck Neoplasms - drug therapy; Head and Neck Neoplasms - enzymology; Head and Neck Neoplasms - pathology; Humans -; Hydroxycholesterols - pharmacology; Immunohistochemistry -