Medizinische Universität Graz - Research portal
Selected Publication:
SHR Neuro Cancer Cardio Lipid Metab Microb
Gangl, K; Reininger, R; Bernhard, D; Campana, R; Pree, I; Reisinger, J; Kneidinger, M; Kundi, M; Dolznig, H; Thurnher, D; Valent, P; Chen, KW; Vrtala, S; Spitzauer, S; Valenta, R; Niederberger, V.
Cigarette smoke facilitates allergen penetration across respiratory epithelium.
Allergy. 2009; 64(3):398-405
Doi: 10.1111/j.1398-9995.2008.01861.x
Web of Science
PubMed
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- Co-authors Med Uni Graz
- Thurnher Dietmar
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- Abstract:
- The association between cigarette smoke exposure and allergic airway disease is a matter for debate. We sought to investigate in an in vitro system whether active smoking reduces the integrity and barrier function of the respiratory epithelium and thus facilitates allergen penetration. We cultured the human bronchial epithelial cell line 16HBE14o- in a transwell culture system as a surrogate for the intact respiratory epithelium. The cell monolayer was exposed to standardized cigarette smoke extract (CSE). The extent and effects of trans-epithelial allergen penetration were measured using 125I-labelled purified major respiratory allergens (rBet v 1, rPhl p 5 and rDer p 2) and histamine release experiments. Exposure of cells to concentrations of CSE similar to those found in smokers induced the development of para-cellular gaps and a decrease in trans-epithelial resistance. CSE exposure induced a more than threefold increase in allergen penetration. Increased subepithelial allergen concentrations provoked a substantial augmentation of histamine release from sensitized basophils. Our results indicate that cigarette smoke is a potent factor capable of reducing the barrier function of the respiratory epithelium for allergens and may contribute to increased allergic inflammation, exacerbation of allergic disease and boosting of IgE memory.
- Find related publications in this database (using NLM MeSH Indexing)
- Allergens - immunology Allergens - metabolism
- Cell Line -
- Cell Membrane - drug effects Cell Membrane - metabolism
- Cell Membrane Permeability - drug effects Cell Membrane Permeability - immunology
- Cytokines -
- Flow Cytometry -
- Histamine Release - drug effects
- Humans -
- Immunohistochemistry -
- Respiratory Mucosa - immunology Respiratory Mucosa - metabolism
- Smoke -
- Tobacco - adverse effects
- Find related publications in this database (Keywords)
- allergen penetration
- cigarette smoke
- permeability
- recombinant allergens
- respiratory epithelium