Medizinische Universität Graz Austria/Österreich - Forschungsportal - Medical University of Graz
Gewählte Publikation:
SHR Neuro Krebs Kardio Lipid Stoffw Microb
Stein, E; Blaimauer, K; Bauer, S; Erovic, BM; Turhani, D; Thurnher, D.
High expression of integrin beta1 correlates with high proliferation capacity in oral keratinocytes.
Wien Klin Wochenschr. 2007; 119(9-10):318-322
Doi: 10.1007/s00508-007-0783-6
Web of Science
PubMed
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- Co-Autor*innen der Med Uni Graz
- Thurnher Dietmar
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- Abstract:
- Large defects of the oral mucosa are still a major challenge in reconstructive surgery. For the development of oral mucosal grafts, successful enrichment of cells with a high proliferative potential is highly desirable. Therefore, the aim of this study was to separate two fractions of oral keratinocytes based on their affinity to collagen type IV. Oral keratinocytes were isolated from oral mucosa and separated into two fractions with different affinities to collagen type IV. Growth curves, Western blot analysis and immunohistochemical staining were used to detect differences between the two cell fractions. The cell fraction (RAC-IV) that adhered to collagen type IV within 20 min showed higher proliferative potential, significantly higher (P < 0.05) expression of integrin beta1 and fewer apoptotic cells. In particular, this fraction included small proliferating cells with the typical polygonal shape of oral keratinocytes, whereas the non-proliferating cells (RAC-IV-D) were irregularly shaped. Immunohistochemical staining showed only some apoptotic RAC-IV cells, whereas RAC-IV-D cells showed a significant increase of M30-positive cells. In addition, Western blotting revealed significantly higher (P < 0.05) expression of integrin beta1 in the RAC-IV fraction than in the RAC-IV-D fraction. Our results show that it is possible to enrich a fraction of highly proliferative oral keratinocytes by means of their high affinity to collagen type IV.
- Find related publications in this database (using NLM MeSH Indexing)
- Adolescent -
- Adult -
- Aged -
- Antigens, CD29 - analysis
- Blotting, Western -
- Cell Count -
- Cell Fractionation -
- Cell Proliferation -
- Cells, Cultured -
- Child -
- Child, Preschool -
- Collagen Type IV - metabolism
- Humans -
- Immunohistochemistry -
- Keratinocytes - cytology
- Keratinocytes - metabolism
- Middle Aged -
- Mouth Mucosa - cytology
- Mouth Mucosa - transplantation
- Staining and Labeling -
- Time Factors -
- Tissue Engineering -
- Find related publications in this database (Keywords)
- Mucosa
- oral keratinocytes
- integrin beta 1
- tissue engineering