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SHR Neuro Cancer Cardio Lipid Metab Microb
Stein, E; Koehn, J; Sutter, W; Wendtlandt, G; Wanschitz, F; Thurnher, D; Baghestanian, M; Turhani, D.
Initial effects of low-level laser therapy on growth and differentiation of human osteoblast-like cells.
Wien Klin Wochenschr. 2008; 120(3-4):112-117
Doi: 10.1007/s00508-008-0932-6
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- Co-authors Med Uni Graz
- Thurnher Dietmar
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- Abstract:
- Low-level laser therapy is a clinically well established tool for enhancement of wound healing. In vitro studies have also shown that low level laser therapy has a biostimulatory effect on cells of different origin. The aim of this in vitro study was to investigate the initial effect of low-level laser therapy on growth and differentiation of human osteoblast-like cells. SaOS-2 cells were irradiated with laser doses of 1 J/cm2 and 2 J/cm2 using a diode laser with 670 nm wave length and an output power of 400 mW. Untreated cells were used as controls. At 24 h, 48 h and 72 h post irradiation, cells were collected and assayed for viability of attached cells and alkaline phosphatase specific activity. In addition, mRNA expression levels of osteopontin and collagen type I were assessed using semi-quantitative RT-PCR. Over the observation period, cell viability, alkaline phosphatase activity and the expression of osteopontin and collagen type I mRNA were slightly enhanced in cells irradiated with 1 J/cm2 compared with untreated control cells. Increasing the laser dose to 2 J/cm2 reduced cell viability during the first 48 h and resulted in persistently lower alkaline phosphatase activity compared with the other two groups. The expression of osteopontin and collagen type I mRNA slightly decreased with time in untreated controls and cells irradiated with 1 J/cm2, but their expression was increased by treatment with 2 J/cm2 after 72 h. These results indicate that low-level laser therapy has a biostimulatory effect on human osteoblast-like cells during the first 72 h after irradiation. Further studies are needed to determine the potential of low-level laser therapy as new treatment concept in bone regeneration.
- Find related publications in this database (using NLM MeSH Indexing)
- Alkaline Phosphatase - metabolism
- Cell Differentiation - radiation effects
- Cell Division - radiation effects
- Cell Line -
- Cell Survival - radiation effects
- Collagen Type I - genetics
- Gene Expression - radiation effects
- Humans -
- In Vitro Techniques -
- Lasers, Semiconductor -
- Low-Level Light Therapy -
- Osteoblasts - radiation effects
- Osteopontin - genetics
- RNA, Messenger - genetics
- Radiotherapy Dosage -
- Receptor-Like Protein Tyrosine Phosphatases, Class 3 -
- Reverse Transcriptase Polymerase Chain Reaction -
- Find related publications in this database (Keywords)
- LLLT
- SaOS-2
- osteoblast-like cells
- diode laser